Coverart for item
The Resource Angiotensin protocols, edited by Donna H. Wang, (electronic book)

Angiotensin protocols, edited by Donna H. Wang, (electronic book)

Label
Angiotensin protocols
Title
Angiotensin protocols
Statement of responsibility
edited by Donna H. Wang
Contributor
Subject
Genre
Language
eng
Member of
Cataloging source
COO
Dewey number
612/.015756
Illustrations
illustrations
Index
index present
LC call number
QP572.A54
LC item number
A545 2001
Literary form
non fiction
NAL call number
QP572.A54
NAL item number
A545 2001
Nature of contents
  • dictionaries
  • bibliography
http://library.link/vocab/relatedWorkOrContributorName
Wang, Donna H.
Series statement
Methods in molecular medicine
Series volume
51
http://library.link/vocab/subjectName
  • Angiotensins
  • MEDICAL
  • Wetenschappelijke technieken
Label
Angiotensin protocols, edited by Donna H. Wang, (electronic book)
Instantiates
Publication
Bibliography note
Includes bibliographical references and index
Contents
pt. 1. Introduction to the renin-angiotensin system -- Historical perspective to the renin-angiotensin system / J.E. Hall -- Three angiotensin paradigms in one patient: etiology of hypertension, glomerular hemodynamics, and long-term glomerular protection / C.L. Laffer -- pt. 2. Transgenic and gene targeting methods -- Production and use of chimeric mice / J. Takaya -- Genetic manipulation of the renin-angiotensin system using cre-loxP-recombinase / Curt D. Sigmund -- Systemic delivery of a transgene in intact animals by a retroviral vector / M.J. Katovich -- Antisense inhibition of the renin-angiotensin system / D. Mohuczy -- pt. 3. Analysis of transcriptional and translational mechanisms -- Analysis of transcriptional control mechanisms I: techniques for characterization of cis-regulatory elements / C.T. Sherman -- Analysis of transcriptional control mechanisms II: techniques for characterization of trans-acting factors / A.R. Brasier -- Analysis of cytosolic proteins that bind to the 5' leader sequence of the angiotensin AT1 receptor by RNA electromobility shift assay / Z. Wu -- In vitro translation of the angiotensin AT1 receptors in wheat-germ extracts / H. Ji -- pt. 4. Assays for determination of mRNA levels -- Quantitation of angiotensin II receptors by competitive reverse-transcription polymerase chain reaction / W. Wu -- Analysis of RNA by Northern-blot hybridization / P.E. Gallagher -- Use of the ribonuclease protection assay for the analysis and characterization of target mRNAs / S.C. Supowit -- In situ hybridization / S. Riggs Runge -- pt. 5. Assays for determination of protein contents and enzyme activity -- Immunohistochemical detection of angiotensin II receptor / Z.-Q. Wang -- Western blotting / N.R. Madamanchi -- Quantification of angiotensin-converting enzyme (ACE) activity / Q.C. Meng -- Quantification of angiotensin peptides / Q.C. Meng -- Radiolabeling of angiotensin peptides / R.C. Speth -- Assays for radiolabel-photoaffinity labeling of angiotensin receptors / J. Perodin -- Radioligand binding assay / G. Thibault -- Autoradiographic localization and quantification of components of the renin-angiotensin system in tissues / S.Y. Chai -- pt. 6. Biochemical studies of the renin-angiotensin system -- Measurement of intracellular free calcium ion concentration in vascular smooth muscle cells: fluorescence imaging of cytosolic calcium / R.M. Touyz -- Measurement of phospholipase D activation in vascular smooth muscle cells / R.M. Touyz -- Measurement of cylic AMP and cyclic GMP in Xenopus oocytes stimulated with angiotensin II and atrial natriuretic factor / H. Ji -- Measurement of nitric oxide formation / C. Thorup -- Measuring renin secretion from juxtaglomerular cells / A. Kurtz -- pt. 7. Functional studies of the renin-angiotensin system -- Measurement of regional blood flow in the kidney using laser-Doppler flowmetry / R.J. Roman -- Measurement of renal tubular angiotensin II / J.D. Imig -- The in vitro blood-perfused juxtamedullary nephron technique / E.W. Inscho -- In vitro proximal tubule perfusion preparation / J.L. Garvin -- Isolated resistance artery preparation / J.P.M. Wesselman -- Measurement of vascular density / A.S. Greene -- Functional studies of the cerebral circulation / J.H. Lombard
Control code
SPR170169579
Dimensions
unknown
Extent
1 online resource (xiv, 530 p.)
Form of item
online
Isbn
9786610821662
Other physical details
ill.
Specific material designation
remote
Label
Angiotensin protocols, edited by Donna H. Wang, (electronic book)
Publication
Bibliography note
Includes bibliographical references and index
Contents
pt. 1. Introduction to the renin-angiotensin system -- Historical perspective to the renin-angiotensin system / J.E. Hall -- Three angiotensin paradigms in one patient: etiology of hypertension, glomerular hemodynamics, and long-term glomerular protection / C.L. Laffer -- pt. 2. Transgenic and gene targeting methods -- Production and use of chimeric mice / J. Takaya -- Genetic manipulation of the renin-angiotensin system using cre-loxP-recombinase / Curt D. Sigmund -- Systemic delivery of a transgene in intact animals by a retroviral vector / M.J. Katovich -- Antisense inhibition of the renin-angiotensin system / D. Mohuczy -- pt. 3. Analysis of transcriptional and translational mechanisms -- Analysis of transcriptional control mechanisms I: techniques for characterization of cis-regulatory elements / C.T. Sherman -- Analysis of transcriptional control mechanisms II: techniques for characterization of trans-acting factors / A.R. Brasier -- Analysis of cytosolic proteins that bind to the 5' leader sequence of the angiotensin AT1 receptor by RNA electromobility shift assay / Z. Wu -- In vitro translation of the angiotensin AT1 receptors in wheat-germ extracts / H. Ji -- pt. 4. Assays for determination of mRNA levels -- Quantitation of angiotensin II receptors by competitive reverse-transcription polymerase chain reaction / W. Wu -- Analysis of RNA by Northern-blot hybridization / P.E. Gallagher -- Use of the ribonuclease protection assay for the analysis and characterization of target mRNAs / S.C. Supowit -- In situ hybridization / S. Riggs Runge -- pt. 5. Assays for determination of protein contents and enzyme activity -- Immunohistochemical detection of angiotensin II receptor / Z.-Q. Wang -- Western blotting / N.R. Madamanchi -- Quantification of angiotensin-converting enzyme (ACE) activity / Q.C. Meng -- Quantification of angiotensin peptides / Q.C. Meng -- Radiolabeling of angiotensin peptides / R.C. Speth -- Assays for radiolabel-photoaffinity labeling of angiotensin receptors / J. Perodin -- Radioligand binding assay / G. Thibault -- Autoradiographic localization and quantification of components of the renin-angiotensin system in tissues / S.Y. Chai -- pt. 6. Biochemical studies of the renin-angiotensin system -- Measurement of intracellular free calcium ion concentration in vascular smooth muscle cells: fluorescence imaging of cytosolic calcium / R.M. Touyz -- Measurement of phospholipase D activation in vascular smooth muscle cells / R.M. Touyz -- Measurement of cylic AMP and cyclic GMP in Xenopus oocytes stimulated with angiotensin II and atrial natriuretic factor / H. Ji -- Measurement of nitric oxide formation / C. Thorup -- Measuring renin secretion from juxtaglomerular cells / A. Kurtz -- pt. 7. Functional studies of the renin-angiotensin system -- Measurement of regional blood flow in the kidney using laser-Doppler flowmetry / R.J. Roman -- Measurement of renal tubular angiotensin II / J.D. Imig -- The in vitro blood-perfused juxtamedullary nephron technique / E.W. Inscho -- In vitro proximal tubule perfusion preparation / J.L. Garvin -- Isolated resistance artery preparation / J.P.M. Wesselman -- Measurement of vascular density / A.S. Greene -- Functional studies of the cerebral circulation / J.H. Lombard
Control code
SPR170169579
Dimensions
unknown
Extent
1 online resource (xiv, 530 p.)
Form of item
online
Isbn
9786610821662
Other physical details
ill.
Specific material designation
remote

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