Coverart for item
The Resource Biobanking in the era of the stem cell : a technical and operational guide, Jennifer C. Moore, Michael H. Sheldon, Ronald P. Hart, (electronic book)

Biobanking in the era of the stem cell : a technical and operational guide, Jennifer C. Moore, Michael H. Sheldon, Ronald P. Hart, (electronic book)

Label
Biobanking in the era of the stem cell : a technical and operational guide
Title
Biobanking in the era of the stem cell
Title remainder
a technical and operational guide
Statement of responsibility
Jennifer C. Moore, Michael H. Sheldon, Ronald P. Hart
Creator
Contributor
Subject
Language
eng
Summary
The study of mental health disorders and the genetics behind these disorders can be greatly enhanced by the use of induced pluripotent stem cells (iPSC). Since many mental health disorders develop after puberty, the only way in which to study the genetic mechanism of these diseases previously was through cellular surrogates, such as blood or cultured fibroblasts. Having the ability to reprogram adult cells to the pluripotent stage provides the capacity to study the onset of these disorders during a culture model of neural development and to include the impact of genetic risk factors and potential environmental triggers. Working with the National Institute of Mental Health (NIMH), the Rutgers Cell and DNA Repository (RUCDR) has begun banking iPSC source cells and converting those source cells into iPSC for distribution to the scientific community. Although initial protocols were developed to reprogram fibroblasts, the ability to reprogram blood cells has several advantages including less invasive collection, less post collection manipulation, and the large number of samples in existing collections. Here, we provide detailed protocols for reprogramming either fibroblasts with retroviral vectors or cryopreserved lymphocytes with Sendai viral vectors. Our goal is to support the discovery of effective treatments for mental health disorders
Member of
Cataloging source
CaBNVSL
http://library.link/vocab/creatorName
Moore, Jennifer C
Dewey number
616.02774
Illustrations
illustrations
Index
no index present
LC call number
QH588.S83
LC item number
M663 2012
Literary form
non fiction
Nature of contents
  • dictionaries
  • abstracts summaries
  • bibliography
http://library.link/vocab/relatedWorkOrContributorName
  • Sheldon, Michael H.
  • Hart, Ronald Philip.
http://library.link/vocab/subjectName
  • Biobanks
  • Stem cells
  • Fibroblasts
  • Lymphocytes
  • Mental illness
  • Biological Specimen Banks
  • Stem Cells
  • Fibroblasts
  • Lymphocytes
  • Mental Disorders
Target audience
  • adult
  • specialized
Label
Biobanking in the era of the stem cell : a technical and operational guide, Jennifer C. Moore, Michael H. Sheldon, Ronald P. Hart, (electronic book)
Instantiates
Publication
Bibliography note
Includes bibliographical references (p. 65-69)
Color
multicolored
Contents
  • Acknowledgments -- 1. Introduction -- 1.1 iPSC for the study of mental health disorders -- 1.2 Building upon a robust genetics repository -- 1.3 Protocols summary -- 1.4 Outlook for the future of iPSC biobanking --
  • 2. Appendix -- 2.1 Mouse embryonic fibroblast (mEF) preparation -- 2.2 Preparing irradiated mEF plates -- 2.3 Fibroblast conversion -- 2.4 PBMC conversion -- 2.5 iPSC colony identification and selection -- 2.6 iPSC expansion --
  • 3. General protocols -- 3.1 Equipment list -- 3.2 Media list -- 3.3 Preparation of gelatin-coated plates -- 3.4 Preparation of BD Matrigel-coated plates -- 3.5 Hemocytometer counting -- 3.6 Manual scraping tool -- 3.7 Identification and manual removal of differentiation -- 3.8 Live alkaline phosphatase staining of iPSC -- 3.9 Dispase dissociation of iPSC -- 3.10 Accutase dissociation of iPSC -- 3.11 Heat inactivation of fetal bovine serum -- 3.12 Thawing and aliquoting sendai virus --
  • References -- Author biographies
Control code
201206SCB002
Dimensions
unknown
Extent
1 electronic text (xi, 71 p.)
File format
multiple file formats
Form of item
online
Isbn
9781615044733
Issn
2168-3980
Other control number
10.4199/C00059ED1V01Y201206SCB002
Other physical details
ill., digital file.
Reformatting quality
access
Specific material designation
remote
System details
System requirements: Adobe Acrobat reader
Label
Biobanking in the era of the stem cell : a technical and operational guide, Jennifer C. Moore, Michael H. Sheldon, Ronald P. Hart, (electronic book)
Publication
Bibliography note
Includes bibliographical references (p. 65-69)
Color
multicolored
Contents
  • Acknowledgments -- 1. Introduction -- 1.1 iPSC for the study of mental health disorders -- 1.2 Building upon a robust genetics repository -- 1.3 Protocols summary -- 1.4 Outlook for the future of iPSC biobanking --
  • 2. Appendix -- 2.1 Mouse embryonic fibroblast (mEF) preparation -- 2.2 Preparing irradiated mEF plates -- 2.3 Fibroblast conversion -- 2.4 PBMC conversion -- 2.5 iPSC colony identification and selection -- 2.6 iPSC expansion --
  • 3. General protocols -- 3.1 Equipment list -- 3.2 Media list -- 3.3 Preparation of gelatin-coated plates -- 3.4 Preparation of BD Matrigel-coated plates -- 3.5 Hemocytometer counting -- 3.6 Manual scraping tool -- 3.7 Identification and manual removal of differentiation -- 3.8 Live alkaline phosphatase staining of iPSC -- 3.9 Dispase dissociation of iPSC -- 3.10 Accutase dissociation of iPSC -- 3.11 Heat inactivation of fetal bovine serum -- 3.12 Thawing and aliquoting sendai virus --
  • References -- Author biographies
Control code
201206SCB002
Dimensions
unknown
Extent
1 electronic text (xi, 71 p.)
File format
multiple file formats
Form of item
online
Isbn
9781615044733
Issn
2168-3980
Other control number
10.4199/C00059ED1V01Y201206SCB002
Other physical details
ill., digital file.
Reformatting quality
access
Specific material designation
remote
System details
System requirements: Adobe Acrobat reader

Library Locations

Processing Feedback ...