Coverart for item
The Resource Cytochrome P450 protocols, edited by Ian R. Phillips, Elizabeth A. Shephard, Paul R. Ortiz de Montellano, (electronic book)

Cytochrome P450 protocols, edited by Ian R. Phillips, Elizabeth A. Shephard, Paul R. Ortiz de Montellano, (electronic book)

Label
Cytochrome P450 protocols
Title
Cytochrome P450 protocols
Statement of responsibility
edited by Ian R. Phillips, Elizabeth A. Shephard, Paul R. Ortiz de Montellano
Contributor
Subject
Genre
Language
eng
Summary
Cytochromes P450 (CYPs) comprise a large superfamily of proteins that are of central importance in the detoxification or activation of a tremendous number of natural and synthetic hydrophobic xenobiotics, including many therapeutic drugs, chemical carcinogens and environmental pollutants. CYPs are important in mediating interactions between an organism and its chemical environment and in the regulation of physiological processes. Cytochrome P450 Protocols, Third Edition focuses on high-throughput methods for the simultaneous analysis of multiple CYPs, substrates or ligands. Although the emphasis is on CYPs of mammalian origin, it reflects an increasing interest in CYPs of bacterial species. Also included are chapters on cytochrome P450 reductase (the redox partner of CYPs) and the flavin-containing monooxygenases (FMOs), and metabolomic and lipidomic approaches for identification of endogenous substrates of CYPs ('de-orphanizing' CYP substrates). Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Cytochrome P450 Protocols, Third Edition provides a wide range of techniques accessible to researchers in fields as diverse as biochemistry, molecular biology, pharmacology, toxicology, environmental biology and genetics
Member of
Cataloging source
GW5XE
Dewey number
572.7
Illustrations
illustrations
Index
index present
LC call number
QP671.P83
LC item number
C98 2013
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
http://library.link/vocab/relatedWorkOrContributorDate
  • 1951-
  • 1950-
http://library.link/vocab/relatedWorkOrContributorName
  • Phillips, Ian R.
  • Shephard, Elizabeth A.
  • Ortiz de Montellano, Paul R.
Series statement
Methods in molecular biology,
Series volume
v. 987
http://library.link/vocab/subjectName
  • Cytochrome P-450
  • Cytochrome P-450
  • Cytochrome P-450 Enzyme System
Label
Cytochrome P450 protocols, edited by Ian R. Phillips, Elizabeth A. Shephard, Paul R. Ortiz de Montellano, (electronic book)
Instantiates
Publication
Antecedent source
unknown
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Color
multicolored
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
  • High-throughput fluorescence assay for cytochrome P450 mechanism-based Inactivators
  • Cesar Kenaan, Haoming Zhang, and Paul F. Hollenberg
  • Identification of endogenous substrates of orphan cytochrome P450 enzymes through the use of untargeted metabolomics approaches
  • Qian Cheng and F. Peter Guengerich
  • Genetic and mass spectrometric tools for elucidating the physiological function(s) of cytochrome P450 enzymes from Mycobacterium tuberculosis
  • Hugues Ouellet [and others]
  • Escherichia coli expression-based approach for porphyrin substitution in heme proteins
  • Michael B. Winter, Joshua J. Woodward, and Michael A. Marletta
  • Expression in Escherichia coli of a cytochrome P450 enzyme with a cobalt protoporphyrin IX porosthetic group
  • Wesley E. Straub [and others]
  • Bioluminescent assays for cytochrome P450 enzymes
  • Nanodiscs in the studies of membrane-bound cytochrome P450 enzymes
  • A. Luthra [and others]
  • Rapid LC-MS drug metabolite profiling using bioreactor particles
  • Linlin Zhao, Besnik Bajrami, and James F. Rusling
  • Fluorescence-based screening of cytochrome P450 activities in intact cells
  • M. Teresa Donato and M. José Gómez-Lechón
  • Screening for cytochrome P450 reactivity with a reporter enzyme
  • Kersten S. Rabe and Christof M. Niemeyer
  • High-throughput fluorescence assay of cytochrome P450 3A4
  • Qian Cheng and F. Peter Guengerich
  • Douglas S. Auld, Henrike Veith, and James J. Cali
  • Targeted protein capture for analysis of electrophile-protein adducts
  • Rebecca E. Connor [and others]
  • DNA shuffling of cytochrome P450 enzymes
  • James B.Y.H. Behrendorff, Wayne A. Johnston, and Elizabeth M.J. Gillam
  • Measurement of P450 difference spectra using intact cells
  • Wayne A. Johnston and Elizabeth M.J. Gillam
  • DNA shuffling of cytochromes P450 for indigoid pigment production
  • Nedeljka N. Rosic
  • P450 oxidoreductase : genotyping, expression, purification of recombinant protein, and activity assessments of wild-type and mutant protein
  • Vishal Agrawal and Walter L. Miller
  • Simultaneous determination of multiple CYP inhibition constants using a cocktail-probe approach
  • LICRED : a versatile drop-in vector for rapid generation of redox-self-sufficient cytochromes P450
  • Federico Sabbadin, Gideon Grogan, and Neil C. Bruce
  • Update on allele nomenclature for human cytochromes P450 and the human cytochrome P450 allele (CYP-allele) nomenclature database
  • Sarah C. Sim and Magnus Ingelman-Sundberg
  • Simultaneous in vivo phenotyping of CYP enzymes
  • Sussan Ghassabian and Michael Murray
  • Detection of regulatory polymorphisms : high-throughput capillary DNase I footprinting
  • Matthew Hancock and Elizabeth A. Shephard
  • Isolation of mouse hepatocytes
  • Mina Edwards [and others]
  • Michael Zientek and Kuresh Youdim
  • Highly efficient SiRNA and gene transfer into hepatocyte-like HepaRG cells and primary human hepatocytes : new means for drug metabolism and toxicity studies
  • Véronique Laurent [and others]
  • High-throughput mass spectrometric cytochrome P450 inhibition screening
  • Kheng B. Lim, Can C. Ozbal, and Daniel B. Kassel
  • Synthesis, characterization, and application of 13c-methyl isocyanide as an NMR probe of heme protein active sites
  • Christopher McCullough [and others]
Control code
SPR832347905
Dimensions
unknown
Edition
3rd ed.
Extent
1 online resource (xii, 319 pages)
File format
unknown
Form of item
online
Isbn
9781627033213
Level of compression
unknown
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other control number
10.1007/978-1-62703-321-3
Other physical details
illustrations (some color).
Quality assurance targets
not applicable
Reformatting quality
unknown
Reproduction note
Electronic resource.
Sound
unknown sound
Specific material designation
remote
Label
Cytochrome P450 protocols, edited by Ian R. Phillips, Elizabeth A. Shephard, Paul R. Ortiz de Montellano, (electronic book)
Publication
Antecedent source
unknown
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Color
multicolored
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
  • High-throughput fluorescence assay for cytochrome P450 mechanism-based Inactivators
  • Cesar Kenaan, Haoming Zhang, and Paul F. Hollenberg
  • Identification of endogenous substrates of orphan cytochrome P450 enzymes through the use of untargeted metabolomics approaches
  • Qian Cheng and F. Peter Guengerich
  • Genetic and mass spectrometric tools for elucidating the physiological function(s) of cytochrome P450 enzymes from Mycobacterium tuberculosis
  • Hugues Ouellet [and others]
  • Escherichia coli expression-based approach for porphyrin substitution in heme proteins
  • Michael B. Winter, Joshua J. Woodward, and Michael A. Marletta
  • Expression in Escherichia coli of a cytochrome P450 enzyme with a cobalt protoporphyrin IX porosthetic group
  • Wesley E. Straub [and others]
  • Bioluminescent assays for cytochrome P450 enzymes
  • Nanodiscs in the studies of membrane-bound cytochrome P450 enzymes
  • A. Luthra [and others]
  • Rapid LC-MS drug metabolite profiling using bioreactor particles
  • Linlin Zhao, Besnik Bajrami, and James F. Rusling
  • Fluorescence-based screening of cytochrome P450 activities in intact cells
  • M. Teresa Donato and M. José Gómez-Lechón
  • Screening for cytochrome P450 reactivity with a reporter enzyme
  • Kersten S. Rabe and Christof M. Niemeyer
  • High-throughput fluorescence assay of cytochrome P450 3A4
  • Qian Cheng and F. Peter Guengerich
  • Douglas S. Auld, Henrike Veith, and James J. Cali
  • Targeted protein capture for analysis of electrophile-protein adducts
  • Rebecca E. Connor [and others]
  • DNA shuffling of cytochrome P450 enzymes
  • James B.Y.H. Behrendorff, Wayne A. Johnston, and Elizabeth M.J. Gillam
  • Measurement of P450 difference spectra using intact cells
  • Wayne A. Johnston and Elizabeth M.J. Gillam
  • DNA shuffling of cytochromes P450 for indigoid pigment production
  • Nedeljka N. Rosic
  • P450 oxidoreductase : genotyping, expression, purification of recombinant protein, and activity assessments of wild-type and mutant protein
  • Vishal Agrawal and Walter L. Miller
  • Simultaneous determination of multiple CYP inhibition constants using a cocktail-probe approach
  • LICRED : a versatile drop-in vector for rapid generation of redox-self-sufficient cytochromes P450
  • Federico Sabbadin, Gideon Grogan, and Neil C. Bruce
  • Update on allele nomenclature for human cytochromes P450 and the human cytochrome P450 allele (CYP-allele) nomenclature database
  • Sarah C. Sim and Magnus Ingelman-Sundberg
  • Simultaneous in vivo phenotyping of CYP enzymes
  • Sussan Ghassabian and Michael Murray
  • Detection of regulatory polymorphisms : high-throughput capillary DNase I footprinting
  • Matthew Hancock and Elizabeth A. Shephard
  • Isolation of mouse hepatocytes
  • Mina Edwards [and others]
  • Michael Zientek and Kuresh Youdim
  • Highly efficient SiRNA and gene transfer into hepatocyte-like HepaRG cells and primary human hepatocytes : new means for drug metabolism and toxicity studies
  • Véronique Laurent [and others]
  • High-throughput mass spectrometric cytochrome P450 inhibition screening
  • Kheng B. Lim, Can C. Ozbal, and Daniel B. Kassel
  • Synthesis, characterization, and application of 13c-methyl isocyanide as an NMR probe of heme protein active sites
  • Christopher McCullough [and others]
Control code
SPR832347905
Dimensions
unknown
Edition
3rd ed.
Extent
1 online resource (xii, 319 pages)
File format
unknown
Form of item
online
Isbn
9781627033213
Level of compression
unknown
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other control number
10.1007/978-1-62703-321-3
Other physical details
illustrations (some color).
Quality assurance targets
not applicable
Reformatting quality
unknown
Reproduction note
Electronic resource.
Sound
unknown sound
Specific material designation
remote

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