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The Resource DNA microarrays for biomedical research : methods and protocols, edited by Martin Dufva, (electronic book)

DNA microarrays for biomedical research : methods and protocols, edited by Martin Dufva, (electronic book)

Label
DNA microarrays for biomedical research : methods and protocols
Title
DNA microarrays for biomedical research
Title remainder
methods and protocols
Statement of responsibility
edited by Martin Dufva
Contributor
Subject
Genre
Language
eng
Summary
DNA microarray technology has revolutionized research in the past decade. Initially an application for mRNA expression studies, the technology now has spread to other applications such as comparative genomic hybridization, SNP and mutation analysis. In DNA Microarrays for Biomedical Research: Methods and Protocols, experts explore these now commonly used applications, addressing probe design strategies, fabrication issues and providing practical examples of detailed methods for generation of high quality DNA microarray data. Chapters incorporate information on some of the largest providers of microarray, including Affymetrix, Ilumina and Agilent, and their use on a variety of applications. Composed in the highly successful Methods in Molecular Biology series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Authoritative and highly practical, DNA Microarrays for Biomedical Research: Methods and Protocols presents a variety of protocols which can be easily reproduced, allowing researchers to gain surprising insight into the complex world of DNA microarray technology
Member of
Cataloging source
CUS
Dewey number
572.8636
Illustrations
illustrations
Index
index present
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
http://library.link/vocab/relatedWorkOrContributorName
Dufva, Martin
http://library.link/vocab/subjectName
  • DNA microarrays
  • Oligonucleotide Array Sequence Analysis
Label
DNA microarrays for biomedical research : methods and protocols, edited by Martin Dufva, (electronic book)
Instantiates
Publication
Bibliography note
Includes bibliographical references and index
Contents
Introduction to microarray technology / Martin Dufva -- Probe design for expression arrays using OligoWiz / Rasmus Wernersson -- Comparative genomic hybridization : microarray design and data interpretation / Richard Redon and Nigel P. Carter -- Design of tag SNP whole genome genotyping arrays / Daniel A. Peiffer and Kevin L. Gunderson -- Fabrication of DNA microarray / Martin Dufva -- Immobilization chemistries / Sascha Todt and Dietmar H. Blohm -- Fabrication using contact spotter / Annelie Waldén and Peter Nilsson -- RNA preparation and characterization for gene expression studies / Michael Stangegaard -- Gene expression analysis using Agilent DNA microarrays / Michael Stangegaard -- Target preparation for genotyping specific genes or gene segments / Jesper Petersen, Lena Poulsen, and Martin Dufva -- Genotyping of mutations in the beta-globin gene using allele specific hybridization / Lena Poulsen, Jesper Petersen, and Martin Dufva -- Microarray temperature optimization using hybridization kinetics / Steve Blair [and others] -- Whole-genome genotyping on bead arrays / Kevin L. Gunderson -- Genotyping single nucleotide polymorphisms by multiplex minisequencing using tag-arrays -- Lili Milani and Ann-Christine Syvänen -- Resequencing arrays for diagnostics of respiratory pathogens / Baochuan Lin and Anthony P. Malanoski -- Comparative genomic hybridization : DNA preparation for microarray fabrication / Richard Redon, Diane Rigler, and Nigel P. Carter -- Comparative genomic hybridization : DNA labeling, hybridization, and detection / Richard Redon, Tomas Fitzgerald, and Nigel P. Carter -- Chromatin immunoprecipitation using microarrays / Mickaël Durand-Dubief and Karl Ekwall
Control code
SPR371900185
Extent
ix, 304 p.
Form of item
  • online
  • electronic
Isbn
9781934115695
Other physical details
ill.
Specific material designation
remote
Label
DNA microarrays for biomedical research : methods and protocols, edited by Martin Dufva, (electronic book)
Publication
Bibliography note
Includes bibliographical references and index
Contents
Introduction to microarray technology / Martin Dufva -- Probe design for expression arrays using OligoWiz / Rasmus Wernersson -- Comparative genomic hybridization : microarray design and data interpretation / Richard Redon and Nigel P. Carter -- Design of tag SNP whole genome genotyping arrays / Daniel A. Peiffer and Kevin L. Gunderson -- Fabrication of DNA microarray / Martin Dufva -- Immobilization chemistries / Sascha Todt and Dietmar H. Blohm -- Fabrication using contact spotter / Annelie Waldén and Peter Nilsson -- RNA preparation and characterization for gene expression studies / Michael Stangegaard -- Gene expression analysis using Agilent DNA microarrays / Michael Stangegaard -- Target preparation for genotyping specific genes or gene segments / Jesper Petersen, Lena Poulsen, and Martin Dufva -- Genotyping of mutations in the beta-globin gene using allele specific hybridization / Lena Poulsen, Jesper Petersen, and Martin Dufva -- Microarray temperature optimization using hybridization kinetics / Steve Blair [and others] -- Whole-genome genotyping on bead arrays / Kevin L. Gunderson -- Genotyping single nucleotide polymorphisms by multiplex minisequencing using tag-arrays -- Lili Milani and Ann-Christine Syvänen -- Resequencing arrays for diagnostics of respiratory pathogens / Baochuan Lin and Anthony P. Malanoski -- Comparative genomic hybridization : DNA preparation for microarray fabrication / Richard Redon, Diane Rigler, and Nigel P. Carter -- Comparative genomic hybridization : DNA labeling, hybridization, and detection / Richard Redon, Tomas Fitzgerald, and Nigel P. Carter -- Chromatin immunoprecipitation using microarrays / Mickaël Durand-Dubief and Karl Ekwall
Control code
SPR371900185
Extent
ix, 304 p.
Form of item
  • online
  • electronic
Isbn
9781934115695
Other physical details
ill.
Specific material designation
remote

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