Coverart for item
The Resource E. coli gene expression protocols, edited by Peter E. Vaillancourt, (electronic book)

E. coli gene expression protocols, edited by Peter E. Vaillancourt, (electronic book)

Label
E. coli gene expression protocols
Title
E. coli gene expression protocols
Statement of responsibility
edited by Peter E. Vaillancourt
Contributor
Subject
Genre
Language
eng
Member of
Additional physical form
Also issued in print.
Cataloging source
COO
Dewey number
572.8/65
Illustrations
illustrations
Index
index present
LC call number
QH450
LC item number
.E15 2003
Literary form
non fiction
NAL call number
QH506
NAL item number
.M45 v. 205
Nature of contents
  • dictionaries
  • bibliography
http://library.link/vocab/relatedWorkOrContributorName
Vaillancourt, Peter E
Series statement
Methods in molecular biology
Series volume
v. 205
http://library.link/vocab/subjectName
  • Gene expression
  • Escherichia coli
  • SCIENCE
  • Electronic books
Label
E. coli gene expression protocols, edited by Peter E. Vaillancourt, (electronic book)
Instantiates
Publication
Bibliography note
Includes bibliographical references and index
Contents
1. Cold-Inducible Promoters for Heterologous Protein Expression / Francois Baneyx and Mirna Mujacic -- 2. Dual-Expression Vectors for Efficient Protein Expression in Both E. coil and Mammalian Cells / Rebecca L. Mullinax ... [et al.] -- 3. A Dual-Expression Vector Allowing Expression in E: coli and P. pastoris, Including New Modifications / Angelika Lueking ... [et al.] -- 4. Purification of Recombinant Proteins from E. coli by Engineered Inteins / Ming-Qun Xu and Thomas C. Evans, Jr. -- 5. Calmodulin as an Affinity Purification Tag / Samu Melkko and Dario Neri -- 6. Calmodulin-Binding Peptide as a Removable Affinity Tag for Protein Purification / Wolfgang Klein -- 7. Maltose-Binding Protein as a Solubility Enhancer / Jeffrey D. Fox and David S. Waugh -- 8. Thioredoxin and Related Proteins as Multifunctional Fusion Tags for Soluble Expression in E. coli / Edward R. LaVallie ... [et al.] -- 9. Discovery of New Fusion Protein Systems Designed to Enhance Solubility in E. coli / Gregory D. Davis and Roger G. Harrison -- 10. Assessment of Protein Folding/Solubility in Live Cells / Rhesa D. Stidham ... [et al.] -- 11. Improving Heterologous Protein Folding via Molecular Chaperone and Foldase co-Expression / Francois Baneyx and Joanne L. Palumbo -- 12. High-Throughput Purification of PolyHis-Tagged Recombinant Fusion Proteins / Thomas Lanio, Albert Jeltsch, and Alfred Pingoud -- 13. Co-Expression of Proteins in E. coil Using Dual Expression Vectors / Karen Johnston and Ronen Marmorstein -- 14. Small-Molecule Affinity-Based Matrices for Rapid Protein Purification / Karin A. Hughes and Jean P. Wiley -- 15. Use of tRNA-Supplemented Host Strains for Expression of Heterologous Genes in E. coli / Carsten-Peter Carstens -- 16. Screening Peptide/Protein Libraries Fused to the X Repressor DNA-Binding Domain in E. coil Cells / Leonardo Marino-Ramirez, Lisa Campbell, and James C. Hu -- 17. Studying Protein-Protein Interactions Using a Bacterial Two-Hybrid System / Simon L. Dove -- 18. Using Bio-Panning of FLITRX Peptide Libraries Displayed on E. coli Cell Surface to Study Protein-Protein Interactions / Zhijian Lu, Edward R. LaVallie, and John M. McCoy -- 19. Use of Inteins for the In Vivo Production of Stable Cyclic Peptide Libraries in E. coli / Ernesto Abel-Santos, Charles P. Scott, and Stephen J. Benkovic -- 20. Hyperphage : Improving Antibody Presentation in Phage Display / Olaf Broders, Frank Breitling, and Stefan Dibel -- 21. Combinatorial Biosynthesis of Novel Carotenoids in E. coli / Gerhard Sandmann -- 22. Using Transcriptional-Based Systems for In Vivo Enzyme Screening / Steven M. Firestine, Frank Salinas, and Stephen J. Benkovic -- 23. Identification of Genes Encoding Secreted Proteins Using Mini-OphoA Mutagenesis / Mary N. Burtnick, Paul J. Brett, and Donald E. Woods
Control code
SPR52434313
Dimensions
unknown
Extent
1 online resource (xi, 347 p.)
Form of item
online
Isbn
9781588290083
Other physical details
ill.
Specific material designation
remote
Label
E. coli gene expression protocols, edited by Peter E. Vaillancourt, (electronic book)
Publication
Bibliography note
Includes bibliographical references and index
Contents
1. Cold-Inducible Promoters for Heterologous Protein Expression / Francois Baneyx and Mirna Mujacic -- 2. Dual-Expression Vectors for Efficient Protein Expression in Both E. coil and Mammalian Cells / Rebecca L. Mullinax ... [et al.] -- 3. A Dual-Expression Vector Allowing Expression in E: coli and P. pastoris, Including New Modifications / Angelika Lueking ... [et al.] -- 4. Purification of Recombinant Proteins from E. coli by Engineered Inteins / Ming-Qun Xu and Thomas C. Evans, Jr. -- 5. Calmodulin as an Affinity Purification Tag / Samu Melkko and Dario Neri -- 6. Calmodulin-Binding Peptide as a Removable Affinity Tag for Protein Purification / Wolfgang Klein -- 7. Maltose-Binding Protein as a Solubility Enhancer / Jeffrey D. Fox and David S. Waugh -- 8. Thioredoxin and Related Proteins as Multifunctional Fusion Tags for Soluble Expression in E. coli / Edward R. LaVallie ... [et al.] -- 9. Discovery of New Fusion Protein Systems Designed to Enhance Solubility in E. coli / Gregory D. Davis and Roger G. Harrison -- 10. Assessment of Protein Folding/Solubility in Live Cells / Rhesa D. Stidham ... [et al.] -- 11. Improving Heterologous Protein Folding via Molecular Chaperone and Foldase co-Expression / Francois Baneyx and Joanne L. Palumbo -- 12. High-Throughput Purification of PolyHis-Tagged Recombinant Fusion Proteins / Thomas Lanio, Albert Jeltsch, and Alfred Pingoud -- 13. Co-Expression of Proteins in E. coil Using Dual Expression Vectors / Karen Johnston and Ronen Marmorstein -- 14. Small-Molecule Affinity-Based Matrices for Rapid Protein Purification / Karin A. Hughes and Jean P. Wiley -- 15. Use of tRNA-Supplemented Host Strains for Expression of Heterologous Genes in E. coli / Carsten-Peter Carstens -- 16. Screening Peptide/Protein Libraries Fused to the X Repressor DNA-Binding Domain in E. coil Cells / Leonardo Marino-Ramirez, Lisa Campbell, and James C. Hu -- 17. Studying Protein-Protein Interactions Using a Bacterial Two-Hybrid System / Simon L. Dove -- 18. Using Bio-Panning of FLITRX Peptide Libraries Displayed on E. coli Cell Surface to Study Protein-Protein Interactions / Zhijian Lu, Edward R. LaVallie, and John M. McCoy -- 19. Use of Inteins for the In Vivo Production of Stable Cyclic Peptide Libraries in E. coli / Ernesto Abel-Santos, Charles P. Scott, and Stephen J. Benkovic -- 20. Hyperphage : Improving Antibody Presentation in Phage Display / Olaf Broders, Frank Breitling, and Stefan Dibel -- 21. Combinatorial Biosynthesis of Novel Carotenoids in E. coli / Gerhard Sandmann -- 22. Using Transcriptional-Based Systems for In Vivo Enzyme Screening / Steven M. Firestine, Frank Salinas, and Stephen J. Benkovic -- 23. Identification of Genes Encoding Secreted Proteins Using Mini-OphoA Mutagenesis / Mary N. Burtnick, Paul J. Brett, and Donald E. Woods
Control code
SPR52434313
Dimensions
unknown
Extent
1 online resource (xi, 347 p.)
Form of item
online
Isbn
9781588290083
Other physical details
ill.
Specific material designation
remote

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