The Resource Enzyme engineering : methods and protocols, edited by James C. Samuelson, (electronic book)
Enzyme engineering : methods and protocols, edited by James C. Samuelson, (electronic book)
Resource Information
The item Enzyme engineering : methods and protocols, edited by James C. Samuelson, (electronic book) represents a specific, individual, material embodiment of a distinct intellectual or artistic creation found in University of Liverpool.This item is available to borrow from 1 library branch.
Resource Information
The item Enzyme engineering : methods and protocols, edited by James C. Samuelson, (electronic book) represents a specific, individual, material embodiment of a distinct intellectual or artistic creation found in University of Liverpool.
This item is available to borrow from 1 library branch.
- Summary
- Whether the pursuit is commercially motivated or purely academic, engineering a novel biological catalyst is an enticing challenge. High-resolution protein structure analysis allows for rational alteration of enzyme function, yet many useful enzyme variants are the product of well-designed selection schemes or screening strategies. Enzyme Engineering: Methods and Protocols provides guidance to investigators wishing to create enzyme variants with desired properties. This detailed volume covers such topics as a simple method for generating site-specific mutations within bacterial chromosomes. It also highlights the engineering of two difference types of rare-cutting endonucleases that show great potential in gene therapy applications: The newest development is the emergence of TAL effector nucleases or TALENs. Chapters describe newly developed technologies in sufficient detail so that each method can be practiced in a standard molecular biology laboratory. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible Enzyme Engineering: Methods and Protocols will be valuable for scientists with a budding interest in protein engineering as well as veterans looking for new approaches to apply in established discovery programs
- Language
- eng
- Extent
- 1 online resource (xi, 252 pages)
- Contents
-
- TAL effector nuclease (TALEN) engineering
- Ting Li and Bing Yang
- In vitro evolution of enzymes
- Misha V. Golynskiy, John C. Haugner III, Aleardo Morelli
- Residue-specific incorporation of unnatural amino acids into proteins in vitro and in vivo
- Amrita Singh-Blom, Randall A. Hughes, and Andrew D. Ellington
- Reconstructing evolutionary adaptive paths for protein engineering
- Megan F. Cole, Vanessa E. Cox, Kelsey L. Gratton, Eric A. Gaucher
- Oligonucleotide recombination enabled site-specific mutagenesis in bacteria
- Bryan M. Swingle
- A Tripartite fusion system for the selection of protein variants with increased stability in vivo
- FX cloning : a versatile high-throughput cloning system for characterization of enzyme variants
- Eric R. Geertsma
- Use of Sulfolobus solfataricus PCNA subunit proteins to direct the assembly of multimeric enzyme complexes
- Hidehiko Hirakawa and Teruyuki Nagamune
- Gene synthesis by assembly of deoxyuridine-containing oligonucleotides
- Romualdas Vaisvila and Jurate Bitinaite
- Protein engineering : single or multiple site-directed mutagenesis
- Pei-Chung Hsieh and Romualdas Vaisvila
- Gene assembly and combinatorial libraries in S. cerevisiae via reiterative recombination
- Nili Ostrov, Laura M. Wingler, and Virginia W. Cornish
- Linda Foit and James C.A. Bardwell
- Promiscuity-based enzyme selection for rational directed evolution experiments
- Sandeep Chakraborty, Renu Minda, Lipika Salaye, Abhaya M. Dandekar
- Rational protein sequence diversification by multi-codon scanning mutagenesis
- Jia Liu and T. Ashton Cropp
- Screening libraries for improved solubility : using E. coli dihydrofolate reductase as a reporter
- Jian-Wei Liu and David L. Ollis
- In vitro directed evolution of enzymes expressed by E. coli in microtiter plates
- Bradley J. Stevenson, Sylvia H.-C. Yip, and David L. Ollis
- Determining enzyme kinetics via isothermal titration calorimetry
- Neil A. Demarse, Marie C. Killian, Lee D. Hansen, Colette F. Quinn
- GFP reporter screens for the engineering of amino acid degrading enzymes from libraries expressed in bacteria
- Olga Paley, Giulia Agnello, Jason Cantor, Tae Hyun Yoo
- Flow cytometric assays for interrogating LAGLIDADG homing endonuclease DNA-binding and cleavage properties
- Sarah K. Baxter, Abigail R. Lambert, Andrew M. Scharenberg
- Isbn
- 9781627032926
- Label
- Enzyme engineering : methods and protocols
- Title
- Enzyme engineering
- Title remainder
- methods and protocols
- Statement of responsibility
- edited by James C. Samuelson
- Language
- eng
- Summary
- Whether the pursuit is commercially motivated or purely academic, engineering a novel biological catalyst is an enticing challenge. High-resolution protein structure analysis allows for rational alteration of enzyme function, yet many useful enzyme variants are the product of well-designed selection schemes or screening strategies. Enzyme Engineering: Methods and Protocols provides guidance to investigators wishing to create enzyme variants with desired properties. This detailed volume covers such topics as a simple method for generating site-specific mutations within bacterial chromosomes. It also highlights the engineering of two difference types of rare-cutting endonucleases that show great potential in gene therapy applications: The newest development is the emergence of TAL effector nucleases or TALENs. Chapters describe newly developed technologies in sufficient detail so that each method can be practiced in a standard molecular biology laboratory. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible Enzyme Engineering: Methods and Protocols will be valuable for scientists with a budding interest in protein engineering as well as veterans looking for new approaches to apply in established discovery programs
- Cataloging source
- SCB
- Dewey number
- 572/.7
- Illustrations
- illustrations
- Index
- index present
- LC call number
- QP601
- LC item number
- .E51575 2013
- Literary form
- non fiction
- Nature of contents
-
- dictionaries
- bibliography
- http://library.link/vocab/relatedWorkOrContributorDate
- 1968-
- http://library.link/vocab/relatedWorkOrContributorName
- Samuelson, James C.
- Series statement
- Methods in molecular biology,
- Series volume
- v. 978
- http://library.link/vocab/subjectName
-
- Enzymes
- Enzymes
- Protein engineering
- Enzymes
- Biomedical Engineering
- Label
- Enzyme engineering : methods and protocols, edited by James C. Samuelson, (electronic book)
- Bibliography note
- Includes bibliographical references and index
- Carrier category
- online resource
- Carrier category code
-
- cr
- Carrier MARC source
- rdacarrier
- Content category
- text
- Content type code
-
- txt
- Content type MARC source
- rdacontent
- Contents
-
- TAL effector nuclease (TALEN) engineering
- Ting Li and Bing Yang
- In vitro evolution of enzymes
- Misha V. Golynskiy, John C. Haugner III, Aleardo Morelli
- Residue-specific incorporation of unnatural amino acids into proteins in vitro and in vivo
- Amrita Singh-Blom, Randall A. Hughes, and Andrew D. Ellington
- Reconstructing evolutionary adaptive paths for protein engineering
- Megan F. Cole, Vanessa E. Cox, Kelsey L. Gratton, Eric A. Gaucher
- Oligonucleotide recombination enabled site-specific mutagenesis in bacteria
- Bryan M. Swingle
- A Tripartite fusion system for the selection of protein variants with increased stability in vivo
- FX cloning : a versatile high-throughput cloning system for characterization of enzyme variants
- Eric R. Geertsma
- Use of Sulfolobus solfataricus PCNA subunit proteins to direct the assembly of multimeric enzyme complexes
- Hidehiko Hirakawa and Teruyuki Nagamune
- Gene synthesis by assembly of deoxyuridine-containing oligonucleotides
- Romualdas Vaisvila and Jurate Bitinaite
- Protein engineering : single or multiple site-directed mutagenesis
- Pei-Chung Hsieh and Romualdas Vaisvila
- Gene assembly and combinatorial libraries in S. cerevisiae via reiterative recombination
- Nili Ostrov, Laura M. Wingler, and Virginia W. Cornish
- Linda Foit and James C.A. Bardwell
- Promiscuity-based enzyme selection for rational directed evolution experiments
- Sandeep Chakraborty, Renu Minda, Lipika Salaye, Abhaya M. Dandekar
- Rational protein sequence diversification by multi-codon scanning mutagenesis
- Jia Liu and T. Ashton Cropp
- Screening libraries for improved solubility : using E. coli dihydrofolate reductase as a reporter
- Jian-Wei Liu and David L. Ollis
- In vitro directed evolution of enzymes expressed by E. coli in microtiter plates
- Bradley J. Stevenson, Sylvia H.-C. Yip, and David L. Ollis
- Determining enzyme kinetics via isothermal titration calorimetry
- Neil A. Demarse, Marie C. Killian, Lee D. Hansen, Colette F. Quinn
- GFP reporter screens for the engineering of amino acid degrading enzymes from libraries expressed in bacteria
- Olga Paley, Giulia Agnello, Jason Cantor, Tae Hyun Yoo
- Flow cytometric assays for interrogating LAGLIDADG homing endonuclease DNA-binding and cleavage properties
- Sarah K. Baxter, Abigail R. Lambert, Andrew M. Scharenberg
- Control code
- SPR828694578
- Extent
- 1 online resource (xi, 252 pages)
- Form of item
- online
- Isbn
- 9781627032926
- Media category
- computer
- Media MARC source
- rdamedia
- Media type code
-
- c
- Other physical details
- illustrations (some color).
- Reproduction note
- Electronic resource.
- Specific material designation
- remote
- Label
- Enzyme engineering : methods and protocols, edited by James C. Samuelson, (electronic book)
- Bibliography note
- Includes bibliographical references and index
- Carrier category
- online resource
- Carrier category code
-
- cr
- Carrier MARC source
- rdacarrier
- Content category
- text
- Content type code
-
- txt
- Content type MARC source
- rdacontent
- Contents
-
- TAL effector nuclease (TALEN) engineering
- Ting Li and Bing Yang
- In vitro evolution of enzymes
- Misha V. Golynskiy, John C. Haugner III, Aleardo Morelli
- Residue-specific incorporation of unnatural amino acids into proteins in vitro and in vivo
- Amrita Singh-Blom, Randall A. Hughes, and Andrew D. Ellington
- Reconstructing evolutionary adaptive paths for protein engineering
- Megan F. Cole, Vanessa E. Cox, Kelsey L. Gratton, Eric A. Gaucher
- Oligonucleotide recombination enabled site-specific mutagenesis in bacteria
- Bryan M. Swingle
- A Tripartite fusion system for the selection of protein variants with increased stability in vivo
- FX cloning : a versatile high-throughput cloning system for characterization of enzyme variants
- Eric R. Geertsma
- Use of Sulfolobus solfataricus PCNA subunit proteins to direct the assembly of multimeric enzyme complexes
- Hidehiko Hirakawa and Teruyuki Nagamune
- Gene synthesis by assembly of deoxyuridine-containing oligonucleotides
- Romualdas Vaisvila and Jurate Bitinaite
- Protein engineering : single or multiple site-directed mutagenesis
- Pei-Chung Hsieh and Romualdas Vaisvila
- Gene assembly and combinatorial libraries in S. cerevisiae via reiterative recombination
- Nili Ostrov, Laura M. Wingler, and Virginia W. Cornish
- Linda Foit and James C.A. Bardwell
- Promiscuity-based enzyme selection for rational directed evolution experiments
- Sandeep Chakraborty, Renu Minda, Lipika Salaye, Abhaya M. Dandekar
- Rational protein sequence diversification by multi-codon scanning mutagenesis
- Jia Liu and T. Ashton Cropp
- Screening libraries for improved solubility : using E. coli dihydrofolate reductase as a reporter
- Jian-Wei Liu and David L. Ollis
- In vitro directed evolution of enzymes expressed by E. coli in microtiter plates
- Bradley J. Stevenson, Sylvia H.-C. Yip, and David L. Ollis
- Determining enzyme kinetics via isothermal titration calorimetry
- Neil A. Demarse, Marie C. Killian, Lee D. Hansen, Colette F. Quinn
- GFP reporter screens for the engineering of amino acid degrading enzymes from libraries expressed in bacteria
- Olga Paley, Giulia Agnello, Jason Cantor, Tae Hyun Yoo
- Flow cytometric assays for interrogating LAGLIDADG homing endonuclease DNA-binding and cleavage properties
- Sarah K. Baxter, Abigail R. Lambert, Andrew M. Scharenberg
- Control code
- SPR828694578
- Extent
- 1 online resource (xi, 252 pages)
- Form of item
- online
- Isbn
- 9781627032926
- Media category
- computer
- Media MARC source
- rdamedia
- Media type code
-
- c
- Other physical details
- illustrations (some color).
- Reproduction note
- Electronic resource.
- Specific material designation
- remote
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<div class="citation" vocab="http://schema.org/"><i class="fa fa-external-link-square fa-fw"></i> Data from <span resource="http://link.liverpool.ac.uk/portal/Enzyme-engineering--methods-and-protocols/jnOr-ErltCk/" typeof="Book http://bibfra.me/vocab/lite/Item"><span property="name http://bibfra.me/vocab/lite/label"><a href="http://link.liverpool.ac.uk/portal/Enzyme-engineering--methods-and-protocols/jnOr-ErltCk/">Enzyme engineering : methods and protocols, edited by James C. Samuelson, (electronic book)</a></span> - <span property="potentialAction" typeOf="OrganizeAction"><span property="agent" typeof="LibrarySystem http://library.link/vocab/LibrarySystem" resource="http://link.liverpool.ac.uk/"><span property="name http://bibfra.me/vocab/lite/label"><a property="url" href="http://link.liverpool.ac.uk/">University of Liverpool</a></span></span></span></span></div>
