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The Resource Gene biotechnology, William Wu ... [et al.]

Gene biotechnology, William Wu ... [et al.]

Label
Gene biotechnology
Title
Gene biotechnology
Statement of responsibility
William Wu ... [et al.]
Contributor
Subject
Genre
Language
eng
Summary
"Written by a team of internationally recognized scientists, the second edition addresses the needs of researchers in academic and commercial environments and serves as a guide for graduate students designing, implementing, and evaluating experimental projects. Presenting many important new methodologies, this unique resource provides you with the tools for success in molecular and cellular biology research today."--BOOK JACKET
Member of
Cataloging source
DLC
http://bibfra.me/vocab/lite/collectionName
Methods in gene biotechnology
Illustrations
illustrations
Index
index present
Literary form
non fiction
Nature of contents
bibliography
http://library.link/vocab/relatedWorkOrContributorName
Wu, William
http://library.link/vocab/subjectName
  • Genetic engineering
  • Molecular biology
  • Genetic Engineering
  • Gene Expression
  • Genomics
  • Molecular Biology
Label
Gene biotechnology, William Wu ... [et al.]
Instantiates
Publication
Note
First ed. published under title: Methods in gene biotechnology. Boca Raton : CRC Press, 1997
Bibliography note
Includes bibliographical references and index
Contents
  • Ch. 1.
  • Strategies for Novel Research Projects.
  • p. 1
  • Ch. 2.
  • Rapid Isolation of Specific cDNAs or Genes by PCR.
  • p. 15
  • Ch. 3.
  • Construction and Screening of Subtracted and Complete Expression cDNA Libraries.
  • p. 31
  • Ch. 4.
  • Subcloning of Genes or DNA Fragments.
  • p. 73
  • Ch. 5.
  • Nonisotopic and Isotopic DNA or RNA Sequencing.
  • p. 99
  • Ch. 6.
  • Information Superhighway and Computer Databases of Nucleic Acids and Proteins.
  • p. 139
  • Ch. 7.
  • Characterization of DNA or Genes by Southern Blot Hybridization.
  • p. 165
  • Ch. 8.
  • Gene Overexpression by Sense RNA in Mammalian Systems.
  • p. 193
  • Ch. 9.
  • Gene Underexpression in Cultured Cells and Animals by Antisense DNA and RNA Strategies.
  • p. 223
  • Ch. 10.
  • Analysis of Gene Expression at the Functional Genomic Level.
  • p. 247
  • Ch. 11.
  • Analysis of Gene Expression at the Proteomic Level.
  • p. 265
  • Ch. 12.
  • Analysis of Cellular DNA or Abundance of mRNA by Radioactivity in Situ Hybridization (RISH).
  • p. 287
  • Ch. 13.
  • Localization of DNA or Abundance of mRNA by Fluorescence in Situ Hybridization (FISH).
  • p. 311
  • Ch. 14.
  • In Situ PCR Hybridization of Low Copy Genes and in Situ RT-PCR Detection of Low Abundance mRNAs.
  • p. 323
  • Ch. 15.
  • Isolation and Characterization of Genes from Genomic DNA Libraries.
  • p. 341
  • Ch. 16.
  • Culture of Mouse Embryonic Stem Cells as a Model Mammalian Cell Line for Gene Expression.
  • p. 365
  • Ch. 17.
  • New Strategies for Gene Knockout.
  • p. 375
  • Ch. 18.
  • Large-Scale Expression and Purification of Recombinant Proteins in Cultured Cells.
  • p. 409
  • Ch. 19.
  • Quantitative Analysis of Functional Genome by Current Real-Time RT-PCR.
  • p. 445
  • Ch. 20.
  • High-Throughput Analysis of Gene Expression by Cutting-Edge Technology - DNA Microarrays (Gene Chips).
  • p. 461
  • Ch. 21.
  • Construction and Screening of Human Antibody Libraries: Using State-of-the-Art Technology - Phage Display.
  • p. 471
  • Ch. 22.
  • Down-Regulation of Gene Expression in Mammalian Systems via Current siRNA Technology.
  • p. 493
  • Index.
  • p. 509
Control code
ocm52288795
Dimensions
24 cm.
Edition
2nd ed.
Extent
523 p.
Isbn
9780849312885
Lccn
2003053183
Other physical details
ill.
Label
Gene biotechnology, William Wu ... [et al.]
Publication
Note
First ed. published under title: Methods in gene biotechnology. Boca Raton : CRC Press, 1997
Bibliography note
Includes bibliographical references and index
Contents
  • Ch. 1.
  • Strategies for Novel Research Projects.
  • p. 1
  • Ch. 2.
  • Rapid Isolation of Specific cDNAs or Genes by PCR.
  • p. 15
  • Ch. 3.
  • Construction and Screening of Subtracted and Complete Expression cDNA Libraries.
  • p. 31
  • Ch. 4.
  • Subcloning of Genes or DNA Fragments.
  • p. 73
  • Ch. 5.
  • Nonisotopic and Isotopic DNA or RNA Sequencing.
  • p. 99
  • Ch. 6.
  • Information Superhighway and Computer Databases of Nucleic Acids and Proteins.
  • p. 139
  • Ch. 7.
  • Characterization of DNA or Genes by Southern Blot Hybridization.
  • p. 165
  • Ch. 8.
  • Gene Overexpression by Sense RNA in Mammalian Systems.
  • p. 193
  • Ch. 9.
  • Gene Underexpression in Cultured Cells and Animals by Antisense DNA and RNA Strategies.
  • p. 223
  • Ch. 10.
  • Analysis of Gene Expression at the Functional Genomic Level.
  • p. 247
  • Ch. 11.
  • Analysis of Gene Expression at the Proteomic Level.
  • p. 265
  • Ch. 12.
  • Analysis of Cellular DNA or Abundance of mRNA by Radioactivity in Situ Hybridization (RISH).
  • p. 287
  • Ch. 13.
  • Localization of DNA or Abundance of mRNA by Fluorescence in Situ Hybridization (FISH).
  • p. 311
  • Ch. 14.
  • In Situ PCR Hybridization of Low Copy Genes and in Situ RT-PCR Detection of Low Abundance mRNAs.
  • p. 323
  • Ch. 15.
  • Isolation and Characterization of Genes from Genomic DNA Libraries.
  • p. 341
  • Ch. 16.
  • Culture of Mouse Embryonic Stem Cells as a Model Mammalian Cell Line for Gene Expression.
  • p. 365
  • Ch. 17.
  • New Strategies for Gene Knockout.
  • p. 375
  • Ch. 18.
  • Large-Scale Expression and Purification of Recombinant Proteins in Cultured Cells.
  • p. 409
  • Ch. 19.
  • Quantitative Analysis of Functional Genome by Current Real-Time RT-PCR.
  • p. 445
  • Ch. 20.
  • High-Throughput Analysis of Gene Expression by Cutting-Edge Technology - DNA Microarrays (Gene Chips).
  • p. 461
  • Ch. 21.
  • Construction and Screening of Human Antibody Libraries: Using State-of-the-Art Technology - Phage Display.
  • p. 471
  • Ch. 22.
  • Down-Regulation of Gene Expression in Mammalian Systems via Current siRNA Technology.
  • p. 493
  • Index.
  • p. 509
Control code
ocm52288795
Dimensions
24 cm.
Edition
2nd ed.
Extent
523 p.
Isbn
9780849312885
Lccn
2003053183
Other physical details
ill.

Library Locations

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      Ashton Street, Liverpool, L69 3DA, GB
      53.418074 -2.967913
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