Coverart for item
The Resource Hematopoietic stem cell protocols, edited by Kevin D. Bunting and Cheng-Kui Qu, (electronic book)

Hematopoietic stem cell protocols, edited by Kevin D. Bunting and Cheng-Kui Qu, (electronic book)

Label
Hematopoietic stem cell protocols
Title
Hematopoietic stem cell protocols
Statement of responsibility
edited by Kevin D. Bunting and Cheng-Kui Qu
Contributor
Editor
Editor
Subject
Genre
Language
eng
Summary
"Includes cutting-edge methods and protocols. Provides step-by-step detail essential for reproducible results. Contains key notes and implementation advice from the experts. Updating and building upon previous editions, Hematopoietic Stem Cell Protocols, Third Edition provides up-to-date protocols from leading stem cell researchers. This in-depth volume presents a clear view of the landscape of assays available to the stem cell researcher working in the growing hematopoietic stem cell (HSC) field. A robust and active field, it is supported by an abundance of innovative mouse models and molecular tools for analysis of phenotypes and functions in mouse and human cells. Understanding more about hematopoietic stem cell biology is integral if these versatile cells are to be applied effectively to treat and cure a wide range of blood diseases. An introductory chapter puts the major contributions of the book into the proper perspective. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Essential for the laboratory-based researcher, Hematopoietic Stem Cell Protocols, Third Edition is a much needed technical resource in the critically important field of hematopoietic stem cell investigation."--Publisher's description
Member of
Cataloging source
GW5XE
Dewey number
572.810724
Illustrations
illustrations
Index
index present
LC call number
QP92
LC item number
.H4534 2014
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
http://library.link/vocab/relatedWorkOrContributorName
  • Bunting, Kevin D.
  • Qu, Cheng-Kui
Series statement
Methods in molecular biology,
Series volume
v. 1185
http://library.link/vocab/subjectName
  • Hematopoietic stem cells
  • Genetic engineering
  • Hematopoietic Stem Cells
  • Cytological Techniques
  • Hematopoietic Stem Cells
Label
Hematopoietic stem cell protocols, edited by Kevin D. Bunting and Cheng-Kui Qu, (electronic book)
Instantiates
Publication
Copyright
Note
Previous edition: 2008
Antecedent source
unknown
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Color
multicolored
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
  • PART I.
  • OVERVIEW
  • 1.
  • The Hematopoietic Stem Cell Landscape
  • Kevin D. Bunting and Cheng-Kui Qu
  • 4.
  • Hematopoietic Stem and Progenitor Cell Mobilization in Mice
  • Jonathan Hoggatt, Tiffany A. Tate, and Louis M. Pelus
  • 5.
  • Cell Cycle Measurement of Mouse Hematopoietic Stem/Progenitor Cells
  • Brahmananda Reddy Chitteti and Edward F. Srour
  • 6.
  • Flow Cytometric Analysis of Signaling and Apoptosis in Hematopoietic Stem Cells
  • Lei Dong and Cheng-Kui Qu
  • PART II.
  • STEM CELL ENRICHMENT AND HETEROGENEITY
  • 2.
  • Investigating the Interaction Between Hematopoietic Stem Cells and Their Niche During Embryonic Development: Optimizing the Isolation of Fetal and Newborn Stem Cells From Liver, Spleen, and Bone Marrow
  • Huimin Cao, Brenda Williams, and Susan K. Nilsson
  • 3.
  • Single-Cell PCR Profiling of Gene Expression in Hematopoiesis
  • José Teles, Tariq Enver, and Cristina Pina
  • 9.
  • DNA Methylation Profiling of Hematopoietic Stem Cells
  • Amber Hogart Begtrup
  • 10.
  • Metabolic Characterization of Hematopoietic Stem Cells
  • Fatih Kocabas, Junke Zheng, Chengcheng Zhang, and Hesham A. Sadek
  • 11.
  • Nanoproteomic Assays on Hematopoietic Stem Cells
  • Heath L. Bradley, Himalee Sabnis, Deborah Pritchett, and Kevin D. Bunting
  • PART III.
  • MOLECULAR AND CELLULAR CHARACTERIZATION
  • 7.
  • Gene Expression Profiling of Hematopoietic Stem Cells (HSCs)
  • Nalini Raghavachari
  • 8.
  • Measuring MicroRNA Expression in Mouse Hematopoietic Stem Cells
  • Wenhuo Hu and Christopher Y. Park
  • 14.
  • Ex Vivo Expansion of Murine and Human Hematopoietic Stem Cells
  • Phuong L. Doan and John P. Chute
  • 15.
  • Using Microfluidics to Investigate Hematopoietic Stem Cell and Microniche Interactions at the Single Cell Level
  • Byungwook Ahn, Zhengqi Wang, David R. Archer, and Wilbur A. Lam
  • PART IV.
  • IN VITRO ASSAYS AND DIFFERENTIATION
  • 12.
  • Hematopoietic Differentiation of Pluripotent Stem Cells in Culture
  • Jason A. Mills, Prasuna Paluru, Mitchell J. Weiss, Paul Gadue, and Deborah L. French
  • 13.
  • Ex Vivo Assays to Study Self-Renewal, Long-Term Expansion, and Leukemic Transformation of Genetically Modified Human Hematopoietic and Patient-Derived Leukemic Stem Cells
  • Pallavi Sontakke, Marco Carretta, Marta Capala, Hein Schepers, and Jan Jacob Schuringa
  • 18.
  • Immunodeficient Mouse Model for Human Hematopoietic Stem Cell Engraftment and Immune System Development
  • Ken-Edwin Aryee, Leonard D. Shultz, and Michael A. Brehm
  • 19.
  • Homing and Migration Assays of Hematopoietic Stem/Progenitor Cells
  • Xi C. He, Zhenrui Li, Rio Sugimura, Jason Ross, Meng Zhao, and Linheng Li
  • PART V.
  • TRANSPLANTATION ASSAYS AND IMAGING ENGRAFTMENT
  • 16.
  • Five-Lineage Clonal Analysis of Hematopoietic Stem/Progenitor Cells
  • Ryo Yamamoto, Yohei Morita, and Hiromitsu Nakauchi
  • 17.
  • Intravital Imaging of Hematopoietic Stem Cells in the Mouse Skull
  • Juwell W. Wu, Judith M. Runnels, and Charles P. Lin
  • 22.
  • High-Throughput Genomic Mapping of Vector Integration Sites in Gene Therapy Studies
  • Brian C. Beard, Jennifer E. Adair, Grant D. Trobridge, and Hans-Peter Kiem
  • 23.
  • Barcoded Vector Libraries and Retroviral or Lentiviral Barcoding of Hematopoietic Stem Cells
  • Leonid V. Bystrykh, Gerald de Haan, and Evgenia Verovskaya
  • PART VI.
  • GENETIC MODIFICATION
  • 20.
  • Retroviral Transduction of Murine and Human Hematopoietic Progenitors and Stem Cells
  • Marioara F. Ciuculescu, Christian Brendel, Chad E. Harris, and David A. Williams
  • 21.
  • Lentiviral Gene Transduction of Mouse and Human Hematopoietic Stem Cells
  • Niek P. van Til and Gerard Wagemaker
Control code
SPR885199906
Dimensions
unknown
Edition
Third Edition.
Extent
1 online resource (xii, 364 pages)
File format
unknown
Form of item
online
Isbn
9781493911325
Level of compression
unknown
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other control number
10.1007/978-1-4939-1133-2
Other physical details
illustrations (some color)
Quality assurance targets
not applicable
Reformatting quality
unknown
Reproduction note
Electronic resource.
Sound
unknown sound
Specific material designation
remote
Label
Hematopoietic stem cell protocols, edited by Kevin D. Bunting and Cheng-Kui Qu, (electronic book)
Publication
Copyright
Note
Previous edition: 2008
Antecedent source
unknown
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Color
multicolored
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
  • PART I.
  • OVERVIEW
  • 1.
  • The Hematopoietic Stem Cell Landscape
  • Kevin D. Bunting and Cheng-Kui Qu
  • 4.
  • Hematopoietic Stem and Progenitor Cell Mobilization in Mice
  • Jonathan Hoggatt, Tiffany A. Tate, and Louis M. Pelus
  • 5.
  • Cell Cycle Measurement of Mouse Hematopoietic Stem/Progenitor Cells
  • Brahmananda Reddy Chitteti and Edward F. Srour
  • 6.
  • Flow Cytometric Analysis of Signaling and Apoptosis in Hematopoietic Stem Cells
  • Lei Dong and Cheng-Kui Qu
  • PART II.
  • STEM CELL ENRICHMENT AND HETEROGENEITY
  • 2.
  • Investigating the Interaction Between Hematopoietic Stem Cells and Their Niche During Embryonic Development: Optimizing the Isolation of Fetal and Newborn Stem Cells From Liver, Spleen, and Bone Marrow
  • Huimin Cao, Brenda Williams, and Susan K. Nilsson
  • 3.
  • Single-Cell PCR Profiling of Gene Expression in Hematopoiesis
  • José Teles, Tariq Enver, and Cristina Pina
  • 9.
  • DNA Methylation Profiling of Hematopoietic Stem Cells
  • Amber Hogart Begtrup
  • 10.
  • Metabolic Characterization of Hematopoietic Stem Cells
  • Fatih Kocabas, Junke Zheng, Chengcheng Zhang, and Hesham A. Sadek
  • 11.
  • Nanoproteomic Assays on Hematopoietic Stem Cells
  • Heath L. Bradley, Himalee Sabnis, Deborah Pritchett, and Kevin D. Bunting
  • PART III.
  • MOLECULAR AND CELLULAR CHARACTERIZATION
  • 7.
  • Gene Expression Profiling of Hematopoietic Stem Cells (HSCs)
  • Nalini Raghavachari
  • 8.
  • Measuring MicroRNA Expression in Mouse Hematopoietic Stem Cells
  • Wenhuo Hu and Christopher Y. Park
  • 14.
  • Ex Vivo Expansion of Murine and Human Hematopoietic Stem Cells
  • Phuong L. Doan and John P. Chute
  • 15.
  • Using Microfluidics to Investigate Hematopoietic Stem Cell and Microniche Interactions at the Single Cell Level
  • Byungwook Ahn, Zhengqi Wang, David R. Archer, and Wilbur A. Lam
  • PART IV.
  • IN VITRO ASSAYS AND DIFFERENTIATION
  • 12.
  • Hematopoietic Differentiation of Pluripotent Stem Cells in Culture
  • Jason A. Mills, Prasuna Paluru, Mitchell J. Weiss, Paul Gadue, and Deborah L. French
  • 13.
  • Ex Vivo Assays to Study Self-Renewal, Long-Term Expansion, and Leukemic Transformation of Genetically Modified Human Hematopoietic and Patient-Derived Leukemic Stem Cells
  • Pallavi Sontakke, Marco Carretta, Marta Capala, Hein Schepers, and Jan Jacob Schuringa
  • 18.
  • Immunodeficient Mouse Model for Human Hematopoietic Stem Cell Engraftment and Immune System Development
  • Ken-Edwin Aryee, Leonard D. Shultz, and Michael A. Brehm
  • 19.
  • Homing and Migration Assays of Hematopoietic Stem/Progenitor Cells
  • Xi C. He, Zhenrui Li, Rio Sugimura, Jason Ross, Meng Zhao, and Linheng Li
  • PART V.
  • TRANSPLANTATION ASSAYS AND IMAGING ENGRAFTMENT
  • 16.
  • Five-Lineage Clonal Analysis of Hematopoietic Stem/Progenitor Cells
  • Ryo Yamamoto, Yohei Morita, and Hiromitsu Nakauchi
  • 17.
  • Intravital Imaging of Hematopoietic Stem Cells in the Mouse Skull
  • Juwell W. Wu, Judith M. Runnels, and Charles P. Lin
  • 22.
  • High-Throughput Genomic Mapping of Vector Integration Sites in Gene Therapy Studies
  • Brian C. Beard, Jennifer E. Adair, Grant D. Trobridge, and Hans-Peter Kiem
  • 23.
  • Barcoded Vector Libraries and Retroviral or Lentiviral Barcoding of Hematopoietic Stem Cells
  • Leonid V. Bystrykh, Gerald de Haan, and Evgenia Verovskaya
  • PART VI.
  • GENETIC MODIFICATION
  • 20.
  • Retroviral Transduction of Murine and Human Hematopoietic Progenitors and Stem Cells
  • Marioara F. Ciuculescu, Christian Brendel, Chad E. Harris, and David A. Williams
  • 21.
  • Lentiviral Gene Transduction of Mouse and Human Hematopoietic Stem Cells
  • Niek P. van Til and Gerard Wagemaker
Control code
SPR885199906
Dimensions
unknown
Edition
Third Edition.
Extent
1 online resource (xii, 364 pages)
File format
unknown
Form of item
online
Isbn
9781493911325
Level of compression
unknown
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other control number
10.1007/978-1-4939-1133-2
Other physical details
illustrations (some color)
Quality assurance targets
not applicable
Reformatting quality
unknown
Reproduction note
Electronic resource.
Sound
unknown sound
Specific material designation
remote

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