Coverart for item
The Resource Human embryonic stem cell protocols, edited by Kursad Turksen, (electronic book)

Human embryonic stem cell protocols, edited by Kursad Turksen, (electronic book)

Label
Human embryonic stem cell protocols
Title
Human embryonic stem cell protocols
Statement of responsibility
edited by Kursad Turksen
Contributor
Subject
Genre
Language
eng
Summary
Despite political and ethical controversies surrounding the study of human embryonic stem (hES) cells, interest remains high in understanding the regulatory mechanisms of stem cell self-renewal, their differentiation along various lineages, and their potential use in regenerative medicine. In Human Embryonic Stem Cell Protocols, internationally respected researchers describe in detail their most useful techniques for the molecular and cellular manipulation of these intriguing cells. This diverse collection of readily reproducible methods has been optimized for the derivation, characterization, and differentiation of hES cells, with special attention given to regenerative medicine applications. The protocols follow the successful Methods in Molecular Biology series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. A companion CD provides color versions of all illustrations in the book. Comprehensive and cutting-edge, Human Embryonic Stem Cell Protocols offers both novice and expert researchers powerful tools essential to understanding the maintenance and differentiation of human embryonic stem cells, as well as their applications in regenerative medicine today
Member of
Cataloging source
GW5XE
Dewey number
616/.02774
Illustrations
illustrations
Index
index present
LC call number
QH588.S83
LC item number
H856 2006
Literary form
non fiction
NAL call number
QH506
NAL item number
.M45 v. 331
Nature of contents
  • dictionaries
  • bibliography
http://library.link/vocab/relatedWorkOrContributorName
Turksen, Kursad.
Series statement
Methods in molecular biology,
Series volume
331
http://library.link/vocab/subjectName
  • Embryonic stem cells
  • Human embryo
  • Stem Cells
  • Embryo Research
Label
Human embryonic stem cell protocols, edited by Kursad Turksen, (electronic book)
Instantiates
Publication
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Color
mixed
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
Human embryo culture and assessment for the derivation of embryonic stem cells (ESC) / A. Henry Sathananthan and Tiki Osianlis -- Derivation of human embryonic stem cell lines from vitrified human embryos / Teija T. Peura, Julia Schaft, and Tomas Stojanov -- Growth of human embryonic stem cells using derivates of human fibroblasts / Carmen Escobedo-Lucea and miodrag Stojkovic -- In vitro neural differentiation of human embronic stem cells using a low-density mouse embryonic fibroblast feeder protocol / John A. Ozolek ... [et al.] -- Optimization of physiological xenofree molecularly defined media and matrices to maintain human embryonic stem cell pluripotency / Isabelle Pfeiffer ... [et al.] -- Serum-free and feeder-free culture expansion of human embryonic stem cells / Katherine E. Wagner and Mohan C. Vemuri -- Single cell enzymatic dissociation of human embryonic stem cells : a straightforward, robust, and standardized culture method / Catharina Ellerström, Johan Hyllner, and Raimund Strehl -- Monitoring stemness in long-term hESC cultures by real-time PCR / Amparo Galán and Carlos Simón -- Culture and preparation of human embryonic stem cells for proteomics-based applications / Charles C. King -- A two- and three-dimensional approach for visualizing human embryonic stem cell differentiation / Christian B. Brøchner ... [et al.] -- Immunofluorescence and mRNA analysis of human embryonic stem cells (hESCs) grown under feeder-free conditions / Aashir Awan ... [et al.] -- Study of gap junctions in human embryonic stem cells / Raymond C.B. Wong and Alice Pébay -- hESC engineering by integrase-mediated chromosomal targeting / Ying Liu ... [et al.] -- Human embryonic stem cell differentiation on periodontal ligament fibroblasts / Y. Murat Elc̦in, Bülend İnanc̦, and A. Eser Elc̦in -- Generation of neural crest cells and peripheral sensory neurons from human embryonic stem cells / Ronald S. Goldstein ... [et al.] -- Embryonic stem cells as a model for studying melanocyte development / Susan E. Zabierowski and Meenhard Herlyn -- In vitro derivation of chondrogenic cells from human embryonic stem cells / Wei Seong Toh ... [et al.] -- Vascular differentiation of human embryonic stem cells in bioactive hydrogel-based scaffolds / Sharon Gerecht, Lino S. Ferreira, and Robert Langer -- Differentiation of neural precursors and dopaminergic neurons from human embryonic stem cells / Xiao-Quing Zhang and Su-Chung Zhang -- Transplantation of human embryonic stem cells and derivatives to the chick embryo / Ronald S. Goldstein -- Genetic manipulation of human embryonic stem cells / Silvina Epsztejn-Litman and Rachel Eiges -- Genetic manipulation of human embryonic stem cells in serum and feeder-free media / Stefan R. Braam, Chris Denning, and Christine L. Mummery -- Human-induced pluripotent stem cells : derivation, propagation, and freezing in serum- and feeder layer-free culture conditions / Hossein Baharvand ... [et al.]
Control code
SPR468817193
Dimensions
unknown
Edition
2nd ed.
Extent
1 online resource (xii, 455 p.)
Form of item
online
Isbn
9781607613695
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other physical details
ill. (some col.)
Reproduction note
Electronic resource.
Sound
unknown sound
Specific material designation
remote
System details
Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002.
Label
Human embryonic stem cell protocols, edited by Kursad Turksen, (electronic book)
Publication
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Color
mixed
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
Human embryo culture and assessment for the derivation of embryonic stem cells (ESC) / A. Henry Sathananthan and Tiki Osianlis -- Derivation of human embryonic stem cell lines from vitrified human embryos / Teija T. Peura, Julia Schaft, and Tomas Stojanov -- Growth of human embryonic stem cells using derivates of human fibroblasts / Carmen Escobedo-Lucea and miodrag Stojkovic -- In vitro neural differentiation of human embronic stem cells using a low-density mouse embryonic fibroblast feeder protocol / John A. Ozolek ... [et al.] -- Optimization of physiological xenofree molecularly defined media and matrices to maintain human embryonic stem cell pluripotency / Isabelle Pfeiffer ... [et al.] -- Serum-free and feeder-free culture expansion of human embryonic stem cells / Katherine E. Wagner and Mohan C. Vemuri -- Single cell enzymatic dissociation of human embryonic stem cells : a straightforward, robust, and standardized culture method / Catharina Ellerström, Johan Hyllner, and Raimund Strehl -- Monitoring stemness in long-term hESC cultures by real-time PCR / Amparo Galán and Carlos Simón -- Culture and preparation of human embryonic stem cells for proteomics-based applications / Charles C. King -- A two- and three-dimensional approach for visualizing human embryonic stem cell differentiation / Christian B. Brøchner ... [et al.] -- Immunofluorescence and mRNA analysis of human embryonic stem cells (hESCs) grown under feeder-free conditions / Aashir Awan ... [et al.] -- Study of gap junctions in human embryonic stem cells / Raymond C.B. Wong and Alice Pébay -- hESC engineering by integrase-mediated chromosomal targeting / Ying Liu ... [et al.] -- Human embryonic stem cell differentiation on periodontal ligament fibroblasts / Y. Murat Elc̦in, Bülend İnanc̦, and A. Eser Elc̦in -- Generation of neural crest cells and peripheral sensory neurons from human embryonic stem cells / Ronald S. Goldstein ... [et al.] -- Embryonic stem cells as a model for studying melanocyte development / Susan E. Zabierowski and Meenhard Herlyn -- In vitro derivation of chondrogenic cells from human embryonic stem cells / Wei Seong Toh ... [et al.] -- Vascular differentiation of human embryonic stem cells in bioactive hydrogel-based scaffolds / Sharon Gerecht, Lino S. Ferreira, and Robert Langer -- Differentiation of neural precursors and dopaminergic neurons from human embryonic stem cells / Xiao-Quing Zhang and Su-Chung Zhang -- Transplantation of human embryonic stem cells and derivatives to the chick embryo / Ronald S. Goldstein -- Genetic manipulation of human embryonic stem cells / Silvina Epsztejn-Litman and Rachel Eiges -- Genetic manipulation of human embryonic stem cells in serum and feeder-free media / Stefan R. Braam, Chris Denning, and Christine L. Mummery -- Human-induced pluripotent stem cells : derivation, propagation, and freezing in serum- and feeder layer-free culture conditions / Hossein Baharvand ... [et al.]
Control code
SPR468817193
Dimensions
unknown
Edition
2nd ed.
Extent
1 online resource (xii, 455 p.)
Form of item
online
Isbn
9781607613695
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other physical details
ill. (some col.)
Reproduction note
Electronic resource.
Sound
unknown sound
Specific material designation
remote
System details
Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002.

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