The Resource Human embryonic stem cell protocols, edited by Kursad Turksen, (electronic book)
Human embryonic stem cell protocols, edited by Kursad Turksen, (electronic book)
Resource Information
The item Human embryonic stem cell protocols, edited by Kursad Turksen, (electronic book) represents a specific, individual, material embodiment of a distinct intellectual or artistic creation found in University of Liverpool.This item is available to borrow from 1 library branch.
Resource Information
The item Human embryonic stem cell protocols, edited by Kursad Turksen, (electronic book) represents a specific, individual, material embodiment of a distinct intellectual or artistic creation found in University of Liverpool.
This item is available to borrow from 1 library branch.
- Summary
- Despite political and ethical controversies surrounding the study of human embryonic stem (hES) cells, interest remains high in understanding the regulatory mechanisms of stem cell self-renewal, their differentiation along various lineages, and their potential use in regenerative medicine. In Human Embryonic Stem Cell Protocols, internationally respected researchers describe in detail their most useful techniques for the molecular and cellular manipulation of these intriguing cells. This diverse collection of readily reproducible methods has been optimized for the derivation, characterization, and differentiation of hES cells, with special attention given to regenerative medicine applications. The protocols follow the successful Methods in Molecular Biology series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. A companion CD provides color versions of all illustrations in the book. Comprehensive and cutting-edge, Human Embryonic Stem Cell Protocols offers both novice and expert researchers powerful tools essential to understanding the maintenance and differentiation of human embryonic stem cells, as well as their applications in regenerative medicine today
- Language
- eng
- Edition
- 3rd ed.
- Extent
- 1 online resource (xiv, 399 pages)
- Contents
-
- Microarray approach to identify the signaling network responsible for self-renewal of human embryonic stem cells
- Noboru Sato and Ali Brivanlou
- Monitoring stemness in long-term hESC sultures by real-time PCR
- Amparo Galan and Carlos Simon
- Study of gap junctions in human embryonic stem cells
- Alice Pebay and Raymond C.B. Wong
- Immunofluorescence microscopy and mRNA analysis of human embryonic stem cells (hESCs) including primary cilia associated signaling pathways
- Maj Linea Vestergaard [and others]
- Analysis of intracellular calcium signaling in human embryonic stem cells
- Adrienn Pentek, Katalin Paszty, and Agota Apati
- Derivation of human embryonic stem cell lines from vitrified human blastocysts
- Genetic manipulation of human embryonic stem cells
- Rachel Eiges
- Genetic modification in human pluripotent stem cells by homologous recombination and CRISPR/Cas9 system
- Haipeng Xue [and others]
- Use of multicolor flow cytometry for isolation of specific cell populations deriving from differentiated human embryonic stem cells
- Isabella Mengarelli, Andrew Fryga, and Tiziano Barberi
- Definitive endoderm differentiation of human embryonic stem cells combined with selective elimination of undifferentiated cells by methionine deprivation
- Tomonori Tsuyama, Nobuaki Shiraki, and Shoen Kume
- Derivation of endothelial cells and pericytes from human pluripotent stem cells
- Sravanti Kusuma and Sharon Gerecht
- Cara K. Bradley [and others]
- Differentiation of human embryonic stem cells on periodontal ligament fibroblasts
- Y. Murat Elcin, Bulend Inanc, and A. Eser Elcin
- Derivation of epithelial cells from human embryonic stem cells as an in vitro model of vocal mucosa
- Vlasta Lungova, Ciara Leydon, and Susan Thibeault
- Human embryonic and hepatic stem cell differentiation visualized in two and three dimensions based on serial sections
- Peter S. Vestentoft [and others]
- Derivation of chondrogenic cells from human embryonic stem cells for cartilage tissue engineering
- Wei Seong Toh and Tong Cao
- Microgrooved surface modulates neuron differentiation in human embryonic stem cells
- David Lu [and others]
- Characterizing pluripotent stem sells using the TaqMan® hPSC scorecardTM panel
- Directed differentiation of human embryonic stem cells into neural progenitors
- Erin Banda and Laura Grabel
- Direct conversion of pluripotent human embryonic stem cells under defined culture conditions into human neuronal or cardiomyocyte cell therapy derivatives
- Xuejun H. Parsons
- Generation of epithelial cell populations from human pluripotent stem cells using a small-molecule inhibitor of Src family kinases
- Joshua A. Selekman, Xiaojun Lian, and Sean P. Palecek
- Dual-SMAD inhibition/WNT activation-based methods to induce neural crest and derivatives from human pluripotent stem cells
- Stuart M. Chambers [and others]
- Accelerated three-dimensional neuroepithelium formation from human embryonic stem cells and its use for quantitative differentiation to human retinal pigment epithelium
- Yu Zhu, Sven Schreiter, and Elly M. Tanaka
- Jeffrey Fergus, Rene Quintanilla, and Uma Lakshmipathy
- Efficient production of photoreceptor precursor cells from human embryonic stem cells
- Anat Yanai [and others]
- Generation of megakaryocytes and platelets from human pluripotent stem cells
- Marjorie Pick
- Simple protocol for the generation of cardiomyocytes from human pluripotent stem cells
- Glen Lester Sequiera, Ashish Mehta, and Winston Shim
- Erratum to:
- Microgrooved surface modulates neuron differentiation in human embryonic stem cells
- David Lu [and others]
- Growth of human pluripotent stem cells using functional human extracellular matrix
- Andres Sanz-Garcia, Miodrag Stojkovic, and Carmen Escobedo-Lucea
- Efficient expansion of dissociated human pluripotent stem cells using a synthetic substrate
- Eihachiro Kawase
- Isbn
- 9781493926671
- Label
- Human embryonic stem cell protocols
- Title
- Human embryonic stem cell protocols
- Statement of responsibility
- edited by Kursad Turksen
- Language
- eng
- Summary
- Despite political and ethical controversies surrounding the study of human embryonic stem (hES) cells, interest remains high in understanding the regulatory mechanisms of stem cell self-renewal, their differentiation along various lineages, and their potential use in regenerative medicine. In Human Embryonic Stem Cell Protocols, internationally respected researchers describe in detail their most useful techniques for the molecular and cellular manipulation of these intriguing cells. This diverse collection of readily reproducible methods has been optimized for the derivation, characterization, and differentiation of hES cells, with special attention given to regenerative medicine applications. The protocols follow the successful Methods in Molecular Biology series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. A companion CD provides color versions of all illustrations in the book. Comprehensive and cutting-edge, Human Embryonic Stem Cell Protocols offers both novice and expert researchers powerful tools essential to understanding the maintenance and differentiation of human embryonic stem cells, as well as their applications in regenerative medicine today
- Cataloging source
- GW5XE
- Dewey number
- 616/.02774
- Illustrations
- illustrations
- Index
- index present
- LC call number
- QH588.S83
- LC item number
- H856 2006
- Literary form
- non fiction
- NAL call number
- QH506
- NAL item number
- .M45 v. 331
- Nature of contents
-
- dictionaries
- bibliography
- http://library.link/vocab/relatedWorkOrContributorName
- Turksen, Kursad
- Series statement
- Methods in molecular biology,
- Series volume
- 331
- http://library.link/vocab/subjectName
-
- Embryonic stem cells
- Human embryo
- Stem Cells
- Embryo Research
- Label
- Human embryonic stem cell protocols, edited by Kursad Turksen, (electronic book)
- Bibliography note
- Includes bibliographical references and index
- Carrier category
- online resource
- Carrier category code
-
- cr
- Carrier MARC source
- rdacarrier
- Color
- mixed
- Content category
- text
- Content type code
-
- txt
- Content type MARC source
- rdacontent
- Contents
-
- Microarray approach to identify the signaling network responsible for self-renewal of human embryonic stem cells
- Noboru Sato and Ali Brivanlou
- Monitoring stemness in long-term hESC sultures by real-time PCR
- Amparo Galan and Carlos Simon
- Study of gap junctions in human embryonic stem cells
- Alice Pebay and Raymond C.B. Wong
- Immunofluorescence microscopy and mRNA analysis of human embryonic stem cells (hESCs) including primary cilia associated signaling pathways
- Maj Linea Vestergaard [and others]
- Analysis of intracellular calcium signaling in human embryonic stem cells
- Adrienn Pentek, Katalin Paszty, and Agota Apati
- Derivation of human embryonic stem cell lines from vitrified human blastocysts
- Genetic manipulation of human embryonic stem cells
- Rachel Eiges
- Genetic modification in human pluripotent stem cells by homologous recombination and CRISPR/Cas9 system
- Haipeng Xue [and others]
- Use of multicolor flow cytometry for isolation of specific cell populations deriving from differentiated human embryonic stem cells
- Isabella Mengarelli, Andrew Fryga, and Tiziano Barberi
- Definitive endoderm differentiation of human embryonic stem cells combined with selective elimination of undifferentiated cells by methionine deprivation
- Tomonori Tsuyama, Nobuaki Shiraki, and Shoen Kume
- Derivation of endothelial cells and pericytes from human pluripotent stem cells
- Sravanti Kusuma and Sharon Gerecht
- Cara K. Bradley [and others]
- Differentiation of human embryonic stem cells on periodontal ligament fibroblasts
- Y. Murat Elcin, Bulend Inanc, and A. Eser Elcin
- Derivation of epithelial cells from human embryonic stem cells as an in vitro model of vocal mucosa
- Vlasta Lungova, Ciara Leydon, and Susan Thibeault
- Human embryonic and hepatic stem cell differentiation visualized in two and three dimensions based on serial sections
- Peter S. Vestentoft [and others]
- Derivation of chondrogenic cells from human embryonic stem cells for cartilage tissue engineering
- Wei Seong Toh and Tong Cao
- Microgrooved surface modulates neuron differentiation in human embryonic stem cells
- David Lu [and others]
- Characterizing pluripotent stem sells using the TaqMan® hPSC scorecardTM panel
- Directed differentiation of human embryonic stem cells into neural progenitors
- Erin Banda and Laura Grabel
- Direct conversion of pluripotent human embryonic stem cells under defined culture conditions into human neuronal or cardiomyocyte cell therapy derivatives
- Xuejun H. Parsons
- Generation of epithelial cell populations from human pluripotent stem cells using a small-molecule inhibitor of Src family kinases
- Joshua A. Selekman, Xiaojun Lian, and Sean P. Palecek
- Dual-SMAD inhibition/WNT activation-based methods to induce neural crest and derivatives from human pluripotent stem cells
- Stuart M. Chambers [and others]
- Accelerated three-dimensional neuroepithelium formation from human embryonic stem cells and its use for quantitative differentiation to human retinal pigment epithelium
- Yu Zhu, Sven Schreiter, and Elly M. Tanaka
- Jeffrey Fergus, Rene Quintanilla, and Uma Lakshmipathy
- Efficient production of photoreceptor precursor cells from human embryonic stem cells
- Anat Yanai [and others]
- Generation of megakaryocytes and platelets from human pluripotent stem cells
- Marjorie Pick
- Simple protocol for the generation of cardiomyocytes from human pluripotent stem cells
- Glen Lester Sequiera, Ashish Mehta, and Winston Shim
- Erratum to:
- Microgrooved surface modulates neuron differentiation in human embryonic stem cells
- David Lu [and others]
- Growth of human pluripotent stem cells using functional human extracellular matrix
- Andres Sanz-Garcia, Miodrag Stojkovic, and Carmen Escobedo-Lucea
- Efficient expansion of dissociated human pluripotent stem cells using a synthetic substrate
- Eihachiro Kawase
- Control code
- SPR918248813
- Dimensions
- unknown
- Edition
- 3rd ed.
- Extent
- 1 online resource (xiv, 399 pages)
- Form of item
- online
- Isbn
- 9781493926671
- Media category
- computer
- Media MARC source
- rdamedia
- Media type code
-
- c
- Other physical details
- illustrations (some color).
- Reproduction note
- Electronic resource.
- Sound
- unknown sound
- Specific material designation
- remote
- Label
- Human embryonic stem cell protocols, edited by Kursad Turksen, (electronic book)
- Bibliography note
- Includes bibliographical references and index
- Carrier category
- online resource
- Carrier category code
-
- cr
- Carrier MARC source
- rdacarrier
- Color
- mixed
- Content category
- text
- Content type code
-
- txt
- Content type MARC source
- rdacontent
- Contents
-
- Microarray approach to identify the signaling network responsible for self-renewal of human embryonic stem cells
- Noboru Sato and Ali Brivanlou
- Monitoring stemness in long-term hESC sultures by real-time PCR
- Amparo Galan and Carlos Simon
- Study of gap junctions in human embryonic stem cells
- Alice Pebay and Raymond C.B. Wong
- Immunofluorescence microscopy and mRNA analysis of human embryonic stem cells (hESCs) including primary cilia associated signaling pathways
- Maj Linea Vestergaard [and others]
- Analysis of intracellular calcium signaling in human embryonic stem cells
- Adrienn Pentek, Katalin Paszty, and Agota Apati
- Derivation of human embryonic stem cell lines from vitrified human blastocysts
- Genetic manipulation of human embryonic stem cells
- Rachel Eiges
- Genetic modification in human pluripotent stem cells by homologous recombination and CRISPR/Cas9 system
- Haipeng Xue [and others]
- Use of multicolor flow cytometry for isolation of specific cell populations deriving from differentiated human embryonic stem cells
- Isabella Mengarelli, Andrew Fryga, and Tiziano Barberi
- Definitive endoderm differentiation of human embryonic stem cells combined with selective elimination of undifferentiated cells by methionine deprivation
- Tomonori Tsuyama, Nobuaki Shiraki, and Shoen Kume
- Derivation of endothelial cells and pericytes from human pluripotent stem cells
- Sravanti Kusuma and Sharon Gerecht
- Cara K. Bradley [and others]
- Differentiation of human embryonic stem cells on periodontal ligament fibroblasts
- Y. Murat Elcin, Bulend Inanc, and A. Eser Elcin
- Derivation of epithelial cells from human embryonic stem cells as an in vitro model of vocal mucosa
- Vlasta Lungova, Ciara Leydon, and Susan Thibeault
- Human embryonic and hepatic stem cell differentiation visualized in two and three dimensions based on serial sections
- Peter S. Vestentoft [and others]
- Derivation of chondrogenic cells from human embryonic stem cells for cartilage tissue engineering
- Wei Seong Toh and Tong Cao
- Microgrooved surface modulates neuron differentiation in human embryonic stem cells
- David Lu [and others]
- Characterizing pluripotent stem sells using the TaqMan® hPSC scorecardTM panel
- Directed differentiation of human embryonic stem cells into neural progenitors
- Erin Banda and Laura Grabel
- Direct conversion of pluripotent human embryonic stem cells under defined culture conditions into human neuronal or cardiomyocyte cell therapy derivatives
- Xuejun H. Parsons
- Generation of epithelial cell populations from human pluripotent stem cells using a small-molecule inhibitor of Src family kinases
- Joshua A. Selekman, Xiaojun Lian, and Sean P. Palecek
- Dual-SMAD inhibition/WNT activation-based methods to induce neural crest and derivatives from human pluripotent stem cells
- Stuart M. Chambers [and others]
- Accelerated three-dimensional neuroepithelium formation from human embryonic stem cells and its use for quantitative differentiation to human retinal pigment epithelium
- Yu Zhu, Sven Schreiter, and Elly M. Tanaka
- Jeffrey Fergus, Rene Quintanilla, and Uma Lakshmipathy
- Efficient production of photoreceptor precursor cells from human embryonic stem cells
- Anat Yanai [and others]
- Generation of megakaryocytes and platelets from human pluripotent stem cells
- Marjorie Pick
- Simple protocol for the generation of cardiomyocytes from human pluripotent stem cells
- Glen Lester Sequiera, Ashish Mehta, and Winston Shim
- Erratum to:
- Microgrooved surface modulates neuron differentiation in human embryonic stem cells
- David Lu [and others]
- Growth of human pluripotent stem cells using functional human extracellular matrix
- Andres Sanz-Garcia, Miodrag Stojkovic, and Carmen Escobedo-Lucea
- Efficient expansion of dissociated human pluripotent stem cells using a synthetic substrate
- Eihachiro Kawase
- Control code
- SPR918248813
- Dimensions
- unknown
- Edition
- 3rd ed.
- Extent
- 1 online resource (xiv, 399 pages)
- Form of item
- online
- Isbn
- 9781493926671
- Media category
- computer
- Media MARC source
- rdamedia
- Media type code
-
- c
- Other physical details
- illustrations (some color).
- Reproduction note
- Electronic resource.
- Sound
- unknown sound
- Specific material designation
- remote
Subject
- Embryo Research
- Embryo Research -- Laboratory Manuals
- Embryonic stem cells -- Laboratory manuals
- Human embryo -- Laboratory manuals
- Stem Cells
- Stem Cells -- Laboratory Manuals
Genre
Member of
- Springer protocols (Series)
- Methods in molecular biology (Clifton, N.J.), v. 584
- Methods in molecular biology (Clifton, N.J.), volume 1307.
- Methods in molecular biology, 584
- Online access with purchase: Springer
- Methods in molecular biology (Clifton, N.J.), v. 331
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<div class="citation" vocab="http://schema.org/"><i class="fa fa-external-link-square fa-fw"></i> Data from <span resource="http://link.liverpool.ac.uk/portal/Human-embryonic-stem-cell-protocols-edited-by/wHrWw7FFkHw/" typeof="Book http://bibfra.me/vocab/lite/Item"><span property="name http://bibfra.me/vocab/lite/label"><a href="http://link.liverpool.ac.uk/portal/Human-embryonic-stem-cell-protocols-edited-by/wHrWw7FFkHw/">Human embryonic stem cell protocols, edited by Kursad Turksen, (electronic book)</a></span> - <span property="potentialAction" typeOf="OrganizeAction"><span property="agent" typeof="LibrarySystem http://library.link/vocab/LibrarySystem" resource="http://link.liverpool.ac.uk/"><span property="name http://bibfra.me/vocab/lite/label"><a property="url" href="http://link.liverpool.ac.uk/">University of Liverpool</a></span></span></span></span></div>
