Coverart for item
The Resource Human embryonic stem cell protocols, edited by Kursad Turksen, (electronic book)

Human embryonic stem cell protocols, edited by Kursad Turksen, (electronic book)

Label
Human embryonic stem cell protocols
Title
Human embryonic stem cell protocols
Statement of responsibility
edited by Kursad Turksen
Contributor
Subject
Genre
Language
eng
Summary
Despite political and ethical controversies surrounding the study of human embryonic stem (hES) cells, interest remains high in understanding the regulatory mechanisms of stem cell self-renewal, their differentiation along various lineages, and their potential use in regenerative medicine. In Human Embryonic Stem Cell Protocols, internationally respected researchers describe in detail their most useful techniques for the molecular and cellular manipulation of these intriguing cells. This diverse collection of readily reproducible methods has been optimized for the derivation, characterization, and differentiation of hES cells, with special attention given to regenerative medicine applications. The protocols follow the successful Methods in Molecular Biology series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. A companion CD provides color versions of all illustrations in the book. Comprehensive and cutting-edge, Human Embryonic Stem Cell Protocols offers both novice and expert researchers powerful tools essential to understanding the maintenance and differentiation of human embryonic stem cells, as well as their applications in regenerative medicine today
Member of
Cataloging source
GW5XE
Dewey number
616/.02774
Illustrations
illustrations
Index
index present
LC call number
QH588.S83
LC item number
H856 2006
Literary form
non fiction
NAL call number
QH506
NAL item number
.M45 v. 331
Nature of contents
  • dictionaries
  • bibliography
http://library.link/vocab/relatedWorkOrContributorName
Turksen, Kursad
Series statement
Methods in molecular biology,
Series volume
331
http://library.link/vocab/subjectName
  • Embryonic stem cells
  • Human embryo
  • Stem Cells
  • Embryo Research
Label
Human embryonic stem cell protocols, edited by Kursad Turksen, (electronic book)
Instantiates
Publication
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Color
mixed
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
  • Microarray approach to identify the signaling network responsible for self-renewal of human embryonic stem cells
  • Noboru Sato and Ali Brivanlou
  • Monitoring stemness in long-term hESC sultures by real-time PCR
  • Amparo Galan and Carlos Simon
  • Study of gap junctions in human embryonic stem cells
  • Alice Pebay and Raymond C.B. Wong
  • Immunofluorescence microscopy and mRNA analysis of human embryonic stem cells (hESCs) including primary cilia associated signaling pathways
  • Maj Linea Vestergaard [and others]
  • Analysis of intracellular calcium signaling in human embryonic stem cells
  • Adrienn Pentek, Katalin Paszty, and Agota Apati
  • Derivation of human embryonic stem cell lines from vitrified human blastocysts
  • Genetic manipulation of human embryonic stem cells
  • Rachel Eiges
  • Genetic modification in human pluripotent stem cells by homologous recombination and CRISPR/Cas9 system
  • Haipeng Xue [and others]
  • Use of multicolor flow cytometry for isolation of specific cell populations deriving from differentiated human embryonic stem cells
  • Isabella Mengarelli, Andrew Fryga, and Tiziano Barberi
  • Definitive endoderm differentiation of human embryonic stem cells combined with selective elimination of undifferentiated cells by methionine deprivation
  • Tomonori Tsuyama, Nobuaki Shiraki, and Shoen Kume
  • Derivation of endothelial cells and pericytes from human pluripotent stem cells
  • Sravanti Kusuma and Sharon Gerecht
  • Cara K. Bradley [and others]
  • Differentiation of human embryonic stem cells on periodontal ligament fibroblasts
  • Y. Murat Elcin, Bulend Inanc, and A. Eser Elcin
  • Derivation of epithelial cells from human embryonic stem cells as an in vitro model of vocal mucosa
  • Vlasta Lungova, Ciara Leydon, and Susan Thibeault
  • Human embryonic and hepatic stem cell differentiation visualized in two and three dimensions based on serial sections
  • Peter S. Vestentoft [and others]
  • Derivation of chondrogenic cells from human embryonic stem cells for cartilage tissue engineering
  • Wei Seong Toh and Tong Cao
  • Microgrooved surface modulates neuron differentiation in human embryonic stem cells
  • David Lu [and others]
  • Characterizing pluripotent stem sells using the TaqMan® hPSC scorecardTM panel
  • Directed differentiation of human embryonic stem cells into neural progenitors
  • Erin Banda and Laura Grabel
  • Direct conversion of pluripotent human embryonic stem cells under defined culture conditions into human neuronal or cardiomyocyte cell therapy derivatives
  • Xuejun H. Parsons
  • Generation of epithelial cell populations from human pluripotent stem cells using a small-molecule inhibitor of Src family kinases
  • Joshua A. Selekman, Xiaojun Lian, and Sean P. Palecek
  • Dual-SMAD inhibition/WNT activation-based methods to induce neural crest and derivatives from human pluripotent stem cells
  • Stuart M. Chambers [and others]
  • Accelerated three-dimensional neuroepithelium formation from human embryonic stem cells and its use for quantitative differentiation to human retinal pigment epithelium
  • Yu Zhu, Sven Schreiter, and Elly M. Tanaka
  • Jeffrey Fergus, Rene Quintanilla, and Uma Lakshmipathy
  • Efficient production of photoreceptor precursor cells from human embryonic stem cells
  • Anat Yanai [and others]
  • Generation of megakaryocytes and platelets from human pluripotent stem cells
  • Marjorie Pick
  • Simple protocol for the generation of cardiomyocytes from human pluripotent stem cells
  • Glen Lester Sequiera, Ashish Mehta, and Winston Shim
  • Erratum to:
  • Microgrooved surface modulates neuron differentiation in human embryonic stem cells
  • David Lu [and others]
  • Growth of human pluripotent stem cells using functional human extracellular matrix
  • Andres Sanz-Garcia, Miodrag Stojkovic, and Carmen Escobedo-Lucea
  • Efficient expansion of dissociated human pluripotent stem cells using a synthetic substrate
  • Eihachiro Kawase
Control code
SPR918248813
Dimensions
unknown
Edition
3rd ed.
Extent
1 online resource (xiv, 399 pages)
Form of item
online
Isbn
9781493926671
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other physical details
illustrations (some color).
Reproduction note
Electronic resource.
Sound
unknown sound
Specific material designation
remote
Label
Human embryonic stem cell protocols, edited by Kursad Turksen, (electronic book)
Publication
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Color
mixed
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
  • Microarray approach to identify the signaling network responsible for self-renewal of human embryonic stem cells
  • Noboru Sato and Ali Brivanlou
  • Monitoring stemness in long-term hESC sultures by real-time PCR
  • Amparo Galan and Carlos Simon
  • Study of gap junctions in human embryonic stem cells
  • Alice Pebay and Raymond C.B. Wong
  • Immunofluorescence microscopy and mRNA analysis of human embryonic stem cells (hESCs) including primary cilia associated signaling pathways
  • Maj Linea Vestergaard [and others]
  • Analysis of intracellular calcium signaling in human embryonic stem cells
  • Adrienn Pentek, Katalin Paszty, and Agota Apati
  • Derivation of human embryonic stem cell lines from vitrified human blastocysts
  • Genetic manipulation of human embryonic stem cells
  • Rachel Eiges
  • Genetic modification in human pluripotent stem cells by homologous recombination and CRISPR/Cas9 system
  • Haipeng Xue [and others]
  • Use of multicolor flow cytometry for isolation of specific cell populations deriving from differentiated human embryonic stem cells
  • Isabella Mengarelli, Andrew Fryga, and Tiziano Barberi
  • Definitive endoderm differentiation of human embryonic stem cells combined with selective elimination of undifferentiated cells by methionine deprivation
  • Tomonori Tsuyama, Nobuaki Shiraki, and Shoen Kume
  • Derivation of endothelial cells and pericytes from human pluripotent stem cells
  • Sravanti Kusuma and Sharon Gerecht
  • Cara K. Bradley [and others]
  • Differentiation of human embryonic stem cells on periodontal ligament fibroblasts
  • Y. Murat Elcin, Bulend Inanc, and A. Eser Elcin
  • Derivation of epithelial cells from human embryonic stem cells as an in vitro model of vocal mucosa
  • Vlasta Lungova, Ciara Leydon, and Susan Thibeault
  • Human embryonic and hepatic stem cell differentiation visualized in two and three dimensions based on serial sections
  • Peter S. Vestentoft [and others]
  • Derivation of chondrogenic cells from human embryonic stem cells for cartilage tissue engineering
  • Wei Seong Toh and Tong Cao
  • Microgrooved surface modulates neuron differentiation in human embryonic stem cells
  • David Lu [and others]
  • Characterizing pluripotent stem sells using the TaqMan® hPSC scorecardTM panel
  • Directed differentiation of human embryonic stem cells into neural progenitors
  • Erin Banda and Laura Grabel
  • Direct conversion of pluripotent human embryonic stem cells under defined culture conditions into human neuronal or cardiomyocyte cell therapy derivatives
  • Xuejun H. Parsons
  • Generation of epithelial cell populations from human pluripotent stem cells using a small-molecule inhibitor of Src family kinases
  • Joshua A. Selekman, Xiaojun Lian, and Sean P. Palecek
  • Dual-SMAD inhibition/WNT activation-based methods to induce neural crest and derivatives from human pluripotent stem cells
  • Stuart M. Chambers [and others]
  • Accelerated three-dimensional neuroepithelium formation from human embryonic stem cells and its use for quantitative differentiation to human retinal pigment epithelium
  • Yu Zhu, Sven Schreiter, and Elly M. Tanaka
  • Jeffrey Fergus, Rene Quintanilla, and Uma Lakshmipathy
  • Efficient production of photoreceptor precursor cells from human embryonic stem cells
  • Anat Yanai [and others]
  • Generation of megakaryocytes and platelets from human pluripotent stem cells
  • Marjorie Pick
  • Simple protocol for the generation of cardiomyocytes from human pluripotent stem cells
  • Glen Lester Sequiera, Ashish Mehta, and Winston Shim
  • Erratum to:
  • Microgrooved surface modulates neuron differentiation in human embryonic stem cells
  • David Lu [and others]
  • Growth of human pluripotent stem cells using functional human extracellular matrix
  • Andres Sanz-Garcia, Miodrag Stojkovic, and Carmen Escobedo-Lucea
  • Efficient expansion of dissociated human pluripotent stem cells using a synthetic substrate
  • Eihachiro Kawase
Control code
SPR918248813
Dimensions
unknown
Edition
3rd ed.
Extent
1 online resource (xiv, 399 pages)
Form of item
online
Isbn
9781493926671
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other physical details
illustrations (some color).
Reproduction note
Electronic resource.
Sound
unknown sound
Specific material designation
remote

Library Locations

Processing Feedback ...