Coverart for item
The Resource Human retroviruses : methods and protocols, edited by Elisa Vicenzi and Guido Poli, Division of Immunology, Transplant, and Infectious Diseases, San Raffaele Scientific Institute, Milano, Italy, (electronic book)

Human retroviruses : methods and protocols, edited by Elisa Vicenzi and Guido Poli, Division of Immunology, Transplant, and Infectious Diseases, San Raffaele Scientific Institute, Milano, Italy, (electronic book)

Label
Human retroviruses : methods and protocols
Title
Human retroviruses
Title remainder
methods and protocols
Statement of responsibility
edited by Elisa Vicenzi and Guido Poli, Division of Immunology, Transplant, and Infectious Diseases, San Raffaele Scientific Institute, Milano, Italy
Contributor
Editor of compilation
Editor
Subject
Genre
Language
eng
Summary
"Human Retroviruses: Methods and Protocols collects key experimental protocols that have provided the basis of the major discoveries of the field. Split into five sections, this detailed volume covers mapping of the HIV life cycle, isolation, co-receptor use, and cell tropism of HIV-1, in vivo quantification of HIV-1, biological aspects of HIV-1, as well as HTLVs. Some articles explore assay and function of accessory genes, largely involving the interface between retroviral and host factors, the extracellular role of Tat and Tax, resembling the function of cytokines, and the biotechnological exploitation of HIV as lentiviral vector to carry foreign genes with therapeutic value. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and authoritative, Human Retroviruses: Methods and Protocols provides state-of-art methodological protocols from world leaders in human retrovirology, essential for any lab working this vital field."--Publisher's description
Member of
Cataloging source
GW5XE
Dewey number
579.2/569
Illustrations
illustrations
Index
index present
LC call number
QR414.5
LC item number
.H858 2014
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
http://library.link/vocab/relatedWorkOrContributorName
  • Vicenzi, Elisa
  • Poli, Guido
Series statement
Methods in Molecular Biology,
Series volume
Volume No. 1087
http://library.link/vocab/subjectName
  • Retroviruses
  • HIV (Viruses)
  • HTLV (Viruses)
  • Retroviridae
  • HIV
  • Deltaretrovirus
  • Biomedicine
  • Immunology
  • Virology
  • Laboratory Medicine
Label
Human retroviruses : methods and protocols, edited by Elisa Vicenzi and Guido Poli, Division of Immunology, Transplant, and Infectious Diseases, San Raffaele Scientific Institute, Milano, Italy, (electronic book)
Instantiates
Publication
Copyright
Antecedent source
unknown
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Color
multicolored
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
  • The Cyclosporin A Washout Assay to Detect HIV-1 Uncoating in Infected Cells
  • Amy E. Hulme and Thomas J. Hope
  • Imaging HIV-1 Nuclear Pre-integration Complexes
  • Anna Cereseto and Mauro Giacca
  • HIV-1 Reverse Transcription
  • Andrea Cimarelli and Jean-Luc Darlix
  • RNase H: Specificity, Mechanisms of Action, and Antiviral Target
  • Karin Moelling, Felix Broecker, and John E. Kerrigan
  • HIV-1 Chromatin, Transcription, and the Regulatory Protein Tat
  • Laurence Colin, Eric Verdin, and Carine Van Lint
  • Part I.
  • HIV-1 Rev Function and RNA Nuclear-Cytoplasmic Export
  • Alan Cochrane
  • HIV-1 Accessory Proteins: Nef
  • Anke Heigele, Daniel Sauter, Jan Münch, and Frank Kirchhoff
  • HIV-1 Accessory Proteins: VpR
  • Richard Y. Zhao and Michael I. Bukrinsky
  • HIV-1 Accessory Proteins: Vpu and Vif
  • Amy Andrew and Klaus Strebel
  • SIVSM/HIV-2 Vpx Proteins: Function and Uses in the Infection of Primary Myeloid Cells
  • Gregory Berger and Andrea Cimarelli
  • Mapping the HIV Life Cycle
  • Imaging of HIV Assembly and Release
  • Barbara Müller and Jacomine Krijnse-Locker
  • Virion Attachment and Entry: HIV gp120 Env Biotinylation, gp120 Env, or Integrin Ligand-Binding Assay
  • Claudia Cicala and James Arthos
  • CryoEM Analysis of Capsid Assembly and Structural Changes Upon Interactions with a Host Restriction Factor, TRIM5[alpha]
  • Gongpu Zhao and Peijun Zhang
  • The Fate of HIV-1 Capsid: A Biochemical Assay for HIV-1 Uncoating
  • Yang Yang, Jeremy Luban, and Felipe Diaz-Griffero
  • HIV Infection of Dendritic Cells
  • Najla Nasr, Andrew Harman, Stuart Turville, and Anthony L. Cunningham
  • Histocultures (Tissue Explants) in Human Retrovirology
  • Anush Arakelyan, Wendy Fitzgerald, Jean-Charles Grivel, Christophe Vanpouille, and Leonid Margolis
  • Part II.
  • Isolation, Co-Receptor Use, and Cell Tropism of HIV-1
  • HIV-1 Isolation from Infected Peripheral Blood Mononuclear Cells
  • Stefania Dispinseri, Elisa Saba, Elisa Vicenzi, Neeltje A. Kootstra, Hanneke Schuitemaker, and Gabriella Scarlatti
  • Determination of HIV-1 Co-receptor Usage
  • Mariangela Cavarelli and Gabriella Scarlatti
  • The Macrophage and HIV: Basic Concepts and Methodologies
  • Suzanne Gartner
  • Part III.
  • In Vivo Quantification of HIV-1
  • Single-Copy Quantification of HIV-1 in Clinical Samples
  • Ann Wiegand and Frank Maldarelli
  • Quantification of Total HIV1-DNA in Peripheral Blood Mononuclear Cells
  • Christine Rouzioux, Adeline Mélard, and Véronique Avéttand-Fénoël
  • Part IV.
  • Biotechnological Aspects of HIV-1
  • HIV-1-Based Lentiviral Vectors
  • Ying Poi Liu and Ben Berkhout
  • Quantification of miRNA by Poly(A)-RT-qPCR Arrays and Verification of Target Sites in HIV-1 Using a One-LTR Infectious Molecular Clone
  • Zachary A. Klase, Laurent Houzet, and Kuan-Teh Jeang
  • Part V.
  • HTLVS
  • Investigating Human T Cell Lymphotropic Retrovirus (HTLV) Tax Function with Molecular and Immunophenotypic Techniques
  • Greta Forlani, Roberto S. Accolla, and Giovanna Tosi
  • Proviral Load Determination of HTLV-1 and HTLV-2 in Patients' Peripheral Blood Mononuclear Cells by Real-Time PCR
  • Claudio Casoli, Elisabetta Pilotti, and Umberto Bertazzoni
  • Quantitative Analysis of Human T-Lymphotropic Virus Type 1 (HTLV-1) Gene Expression Using Nucleo-Cytoplasmic Fractionation and Splice Junction-Specific Real-Time RT-PCR (qRT-PCR)
  • Ilaria Cavallari, Francesca Rende, and Vincenzo Ciminale
Control code
SPR864681043
Dimensions
unknown
Extent
1 online resource (xviii, 345 pages)
File format
unknown
Form of item
online
Isbn
9781627036702
Level of compression
unknown
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other control number
10.1007/978-1-62703-670-2
Other physical details
illustrations (some color).
Quality assurance targets
not applicable
Reformatting quality
unknown
Reproduction note
Electronic resource.
Sound
unknown sound
Specific material designation
remote
Label
Human retroviruses : methods and protocols, edited by Elisa Vicenzi and Guido Poli, Division of Immunology, Transplant, and Infectious Diseases, San Raffaele Scientific Institute, Milano, Italy, (electronic book)
Publication
Copyright
Antecedent source
unknown
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Color
multicolored
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
  • The Cyclosporin A Washout Assay to Detect HIV-1 Uncoating in Infected Cells
  • Amy E. Hulme and Thomas J. Hope
  • Imaging HIV-1 Nuclear Pre-integration Complexes
  • Anna Cereseto and Mauro Giacca
  • HIV-1 Reverse Transcription
  • Andrea Cimarelli and Jean-Luc Darlix
  • RNase H: Specificity, Mechanisms of Action, and Antiviral Target
  • Karin Moelling, Felix Broecker, and John E. Kerrigan
  • HIV-1 Chromatin, Transcription, and the Regulatory Protein Tat
  • Laurence Colin, Eric Verdin, and Carine Van Lint
  • Part I.
  • HIV-1 Rev Function and RNA Nuclear-Cytoplasmic Export
  • Alan Cochrane
  • HIV-1 Accessory Proteins: Nef
  • Anke Heigele, Daniel Sauter, Jan Münch, and Frank Kirchhoff
  • HIV-1 Accessory Proteins: VpR
  • Richard Y. Zhao and Michael I. Bukrinsky
  • HIV-1 Accessory Proteins: Vpu and Vif
  • Amy Andrew and Klaus Strebel
  • SIVSM/HIV-2 Vpx Proteins: Function and Uses in the Infection of Primary Myeloid Cells
  • Gregory Berger and Andrea Cimarelli
  • Mapping the HIV Life Cycle
  • Imaging of HIV Assembly and Release
  • Barbara Müller and Jacomine Krijnse-Locker
  • Virion Attachment and Entry: HIV gp120 Env Biotinylation, gp120 Env, or Integrin Ligand-Binding Assay
  • Claudia Cicala and James Arthos
  • CryoEM Analysis of Capsid Assembly and Structural Changes Upon Interactions with a Host Restriction Factor, TRIM5[alpha]
  • Gongpu Zhao and Peijun Zhang
  • The Fate of HIV-1 Capsid: A Biochemical Assay for HIV-1 Uncoating
  • Yang Yang, Jeremy Luban, and Felipe Diaz-Griffero
  • HIV Infection of Dendritic Cells
  • Najla Nasr, Andrew Harman, Stuart Turville, and Anthony L. Cunningham
  • Histocultures (Tissue Explants) in Human Retrovirology
  • Anush Arakelyan, Wendy Fitzgerald, Jean-Charles Grivel, Christophe Vanpouille, and Leonid Margolis
  • Part II.
  • Isolation, Co-Receptor Use, and Cell Tropism of HIV-1
  • HIV-1 Isolation from Infected Peripheral Blood Mononuclear Cells
  • Stefania Dispinseri, Elisa Saba, Elisa Vicenzi, Neeltje A. Kootstra, Hanneke Schuitemaker, and Gabriella Scarlatti
  • Determination of HIV-1 Co-receptor Usage
  • Mariangela Cavarelli and Gabriella Scarlatti
  • The Macrophage and HIV: Basic Concepts and Methodologies
  • Suzanne Gartner
  • Part III.
  • In Vivo Quantification of HIV-1
  • Single-Copy Quantification of HIV-1 in Clinical Samples
  • Ann Wiegand and Frank Maldarelli
  • Quantification of Total HIV1-DNA in Peripheral Blood Mononuclear Cells
  • Christine Rouzioux, Adeline Mélard, and Véronique Avéttand-Fénoël
  • Part IV.
  • Biotechnological Aspects of HIV-1
  • HIV-1-Based Lentiviral Vectors
  • Ying Poi Liu and Ben Berkhout
  • Quantification of miRNA by Poly(A)-RT-qPCR Arrays and Verification of Target Sites in HIV-1 Using a One-LTR Infectious Molecular Clone
  • Zachary A. Klase, Laurent Houzet, and Kuan-Teh Jeang
  • Part V.
  • HTLVS
  • Investigating Human T Cell Lymphotropic Retrovirus (HTLV) Tax Function with Molecular and Immunophenotypic Techniques
  • Greta Forlani, Roberto S. Accolla, and Giovanna Tosi
  • Proviral Load Determination of HTLV-1 and HTLV-2 in Patients' Peripheral Blood Mononuclear Cells by Real-Time PCR
  • Claudio Casoli, Elisabetta Pilotti, and Umberto Bertazzoni
  • Quantitative Analysis of Human T-Lymphotropic Virus Type 1 (HTLV-1) Gene Expression Using Nucleo-Cytoplasmic Fractionation and Splice Junction-Specific Real-Time RT-PCR (qRT-PCR)
  • Ilaria Cavallari, Francesca Rende, and Vincenzo Ciminale
Control code
SPR864681043
Dimensions
unknown
Extent
1 online resource (xviii, 345 pages)
File format
unknown
Form of item
online
Isbn
9781627036702
Level of compression
unknown
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other control number
10.1007/978-1-62703-670-2
Other physical details
illustrations (some color).
Quality assurance targets
not applicable
Reformatting quality
unknown
Reproduction note
Electronic resource.
Sound
unknown sound
Specific material designation
remote

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