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The Resource Introduction to confocal fluorescence microscopy, Michiel Muller, (electronic book)

Introduction to confocal fluorescence microscopy, Michiel Muller, (electronic book)

Label
Introduction to confocal fluorescence microscopy
Title
Introduction to confocal fluorescence microscopy
Statement of responsibility
Michiel Muller
Creator
Contributor
Subject
Language
eng
Summary
This book provides a comprehensive account of the theory of image formation in a confocal fluorescence microscope as well as a practical guideline to the operation of the instrument, its limitations, and the interpretation of confocal microscopy data. The appendices provide a quick reference to optical theory, microscopy-related formulas and definitions, and Fourier theory
Member of
Additional physical form
Also available in print version.
Cataloging source
CaBNvSL
http://library.link/vocab/creatorName
Muller, Michiel.
Dewey number
502.8/25
Illustrations
illustrations
Index
index present
LC call number
QH224
LC item number
.M83 2006
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
http://library.link/vocab/relatedWorkOrContributorName
Society of Photo-optical Instrumentation Engineers
Series statement
Tutorial texts in optical engineering
Series volume
TT69
http://library.link/vocab/subjectName
Confocal fluorescence microscopy
Target audience
adult
Label
Introduction to confocal fluorescence microscopy, Michiel Muller, (electronic book)
Instantiates
Publication
Note
"SPIE digital library."
Bibliography note
Includes bibliographical references (p. 111-114) and index
Color
black and white
Contents
  • Symbols and abbreviations -- Preface -- Chapter 1 Confocal fluorescence microscopy -- 1.1. The principle -- 1.2. The theory. 1.2.1. The point spread function (PSF); 1.2.2. Validity of approximations; 1.2.3. The PSF in 3D; 1.2.4. Image formation; 1.2.5. The confocal PSF; 1.2.6. The optical transfer function (OTF) -- 1.3. What is the resolution?. 1.3.1. Resolution and the OTF; 1.3.2. Magnification -- 1.4. Optical aberrations. 1.4.1. Chromatic aberration; 1.4.2. Refractive index mismatch
  • Chapter 2. Implementation -- 2.1. The microscope objective. 2.1.1. Magnification, NA and immersion; 2.1.2. Tube length; 2.1.3. Cover glass; 2.1.4. Microscope objective types; 2.1.5. Working distance; 2.1.6. Transmission -- 2.2. Scanning. 2.2.1. Specimen scanning; 2.2.2. Laser scanning; 2.2.3. The Nipkow disk; 2.2.4. Bilateral scanning -- 2.3. The detection pinhole -- 2.4. Fluorophores. 2.4.1. Absorption and fluorescence; 2.4.2. Molecular energy levels; 2.4.3. Absorption; 2.4.4. Vibrational and rotational relaxation; 2.4.5. Emission; 2.4.6. Phosphorescence; 2.4.7. Bleaching; 2.4.8. Fluorescent proteins -- 2.5. The laser
  • Chapter 3. Practical limits -- Chapter 4. Digitization -- 4.1. Discrete sampling of continuous signals -- 4.2. Digitization in the spatial domain -- 4.3. Digitization in the intensity domain
  • Chapter 5. Miscellaneous topics -- 5.1. PSF measurement. 5.1.1. Preparation protocol -- 5.2. Restoration/deconvolution. 5.2.1. The principle; 5.2.2. Practical considerations; 5.2.3. Practical example -- 5.3. The commercial instrument. 5.3.1. Excitation; 5.3.2. Objectives; 5.3.3. Dichroics; 5.3.4. Detection pinhole; 5.3.5. Detection; 5.3.6. Speed; 5.3.7. Software -- 5.4. Related 3D fluorescence techniques. 5.4.1. 4[pi]-microscopy; 5.4.2. Two-photon absorption microscopy
  • Appendix A. Elements of optical theory. A.1. Geometrical optics; A.2. Aberration theory -- Appendix B. Formulae, relations, and definitions. B.1. Optical coordinates; B.2. Focal field distribution; B.3. Classical theory versus full diffraction theory; B.4. Confocal PSF -- Appendix C. Fourier theory. C.1. Properties of the Fourier transform -- Bibliography -- Index
Dimensions
unknown
Edition
2nd ed.
Extent
1 online resource (xvi, 119 p. : ill.)
File format
multiple file formats
Form of item
electronic
Isbn
9780819460431
Other physical details
digital file.
Reformatting quality
access
Reproduction note
Electronic resource.
Specific material designation
remote
System details
System requirements: Adobe Acrobat Reader
Label
Introduction to confocal fluorescence microscopy, Michiel Muller, (electronic book)
Publication
Note
"SPIE digital library."
Bibliography note
Includes bibliographical references (p. 111-114) and index
Color
black and white
Contents
  • Symbols and abbreviations -- Preface -- Chapter 1 Confocal fluorescence microscopy -- 1.1. The principle -- 1.2. The theory. 1.2.1. The point spread function (PSF); 1.2.2. Validity of approximations; 1.2.3. The PSF in 3D; 1.2.4. Image formation; 1.2.5. The confocal PSF; 1.2.6. The optical transfer function (OTF) -- 1.3. What is the resolution?. 1.3.1. Resolution and the OTF; 1.3.2. Magnification -- 1.4. Optical aberrations. 1.4.1. Chromatic aberration; 1.4.2. Refractive index mismatch
  • Chapter 2. Implementation -- 2.1. The microscope objective. 2.1.1. Magnification, NA and immersion; 2.1.2. Tube length; 2.1.3. Cover glass; 2.1.4. Microscope objective types; 2.1.5. Working distance; 2.1.6. Transmission -- 2.2. Scanning. 2.2.1. Specimen scanning; 2.2.2. Laser scanning; 2.2.3. The Nipkow disk; 2.2.4. Bilateral scanning -- 2.3. The detection pinhole -- 2.4. Fluorophores. 2.4.1. Absorption and fluorescence; 2.4.2. Molecular energy levels; 2.4.3. Absorption; 2.4.4. Vibrational and rotational relaxation; 2.4.5. Emission; 2.4.6. Phosphorescence; 2.4.7. Bleaching; 2.4.8. Fluorescent proteins -- 2.5. The laser
  • Chapter 3. Practical limits -- Chapter 4. Digitization -- 4.1. Discrete sampling of continuous signals -- 4.2. Digitization in the spatial domain -- 4.3. Digitization in the intensity domain
  • Chapter 5. Miscellaneous topics -- 5.1. PSF measurement. 5.1.1. Preparation protocol -- 5.2. Restoration/deconvolution. 5.2.1. The principle; 5.2.2. Practical considerations; 5.2.3. Practical example -- 5.3. The commercial instrument. 5.3.1. Excitation; 5.3.2. Objectives; 5.3.3. Dichroics; 5.3.4. Detection pinhole; 5.3.5. Detection; 5.3.6. Speed; 5.3.7. Software -- 5.4. Related 3D fluorescence techniques. 5.4.1. 4[pi]-microscopy; 5.4.2. Two-photon absorption microscopy
  • Appendix A. Elements of optical theory. A.1. Geometrical optics; A.2. Aberration theory -- Appendix B. Formulae, relations, and definitions. B.1. Optical coordinates; B.2. Focal field distribution; B.3. Classical theory versus full diffraction theory; B.4. Confocal PSF -- Appendix C. Fourier theory. C.1. Properties of the Fourier transform -- Bibliography -- Index
Dimensions
unknown
Edition
2nd ed.
Extent
1 online resource (xvi, 119 p. : ill.)
File format
multiple file formats
Form of item
electronic
Isbn
9780819460431
Other physical details
digital file.
Reformatting quality
access
Reproduction note
Electronic resource.
Specific material designation
remote
System details
System requirements: Adobe Acrobat Reader

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