Coverart for item
The Resource Molecular biology techniques : a classroom laboratory manual, by Susan Carson, Heather Miller, D. Scott Witherow, (electronic book)

Molecular biology techniques : a classroom laboratory manual, by Susan Carson, Heather Miller, D. Scott Witherow, (electronic book)

Label
Molecular biology techniques : a classroom laboratory manual
Title
Molecular biology techniques
Title remainder
a classroom laboratory manual
Statement of responsibility
by Susan Carson, Heather Miller, D. Scott Witherow
Creator
Contributor
Subject
Genre
Language
eng
Summary
This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein. The second edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, rather than a 4-week intensive course. The "project" approach to experiments was maintained: students still follow a cloning project through to completion, culminating in the purification of recombinant protein. It takes advantage of the enhanced green fluorescent protein-students can actually visualize positive clones following IPTG induction. *Cover basic concepts and techniques used in molecular biology research labs *Student-tested labs proven successful in a real classroom laboratories *Exercises simulate a cloning project that would be performed in a real research lab *"Project" approach to experiments gives students an overview of the entire process *Prep-list appendix contains necessary recipes and catalog numbers, providing staff with detailed instructions
Member of
Cataloging source
OPELS
http://library.link/vocab/creatorName
Carson, Sue
Dewey number
572.8078
Illustrations
illustrations
Index
index present
LC call number
QH506
LC item number
.C36 2012
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
http://library.link/vocab/relatedWorkOrContributorName
  • Miller, Heather B.
  • Witherow, D. Scott.
http://library.link/vocab/subjectName
  • DNA, Recombinant
  • Molecular Biology
  • Protein Engineering
  • Molecular biology
Label
Molecular biology techniques : a classroom laboratory manual, by Susan Carson, Heather Miller, D. Scott Witherow, (electronic book)
Instantiates
Publication
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Color
multicolored
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
Foreword -- Acknowledgements -- Note to Instructors -- Instrumentation -- Nomenclature -- INTRODUCTION: Conceptual outline for experiments -- PART I MANIPULATION OF DNA -- PART II SCREENING TRANSFORMANTS -- PART III EXPRESSION, DETECTION, AND PURIFICATION OF RECOMBINANT PROTEINS -- Appendices: Equipment, Prep list, Making sense of orientation
Control code
SCIDI776632410
Dimensions
unknown
Edition
3rd ed.
Extent
1 online resource (200 p.)
Form of item
online
Isbn
9780123855442
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other physical details
ill.
Specific material designation
remote
Label
Molecular biology techniques : a classroom laboratory manual, by Susan Carson, Heather Miller, D. Scott Witherow, (electronic book)
Publication
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Color
multicolored
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
Foreword -- Acknowledgements -- Note to Instructors -- Instrumentation -- Nomenclature -- INTRODUCTION: Conceptual outline for experiments -- PART I MANIPULATION OF DNA -- PART II SCREENING TRANSFORMANTS -- PART III EXPRESSION, DETECTION, AND PURIFICATION OF RECOMBINANT PROTEINS -- Appendices: Equipment, Prep list, Making sense of orientation
Control code
SCIDI776632410
Dimensions
unknown
Edition
3rd ed.
Extent
1 online resource (200 p.)
Form of item
online
Isbn
9780123855442
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other physical details
ill.
Specific material designation
remote

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