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The Resource Molecular toxicology protocols, edited by Phouthone Keohavong, Stephen G. Grant, (electronic book)

Molecular toxicology protocols, edited by Phouthone Keohavong, Stephen G. Grant, (electronic book)

Label
Molecular toxicology protocols
Title
Molecular toxicology protocols
Statement of responsibility
edited by Phouthone Keohavong, Stephen G. Grant
Contributor
Editor
Subject
Genre
Language
eng
Summary
Molecular Toxicology Protocols, Second Edition aims to bring together a series of articles describing validated methods to elucidate specific molecular aspects of toxicology, the emphasis being on the application of molecular methods to genetic toxicology. The volume is divided into ten parts, roughly corresponding to the spectrum of biomarkers intermediate between exposure and disease outcomes as proposed in molecular epidemiology models. Subjects of these new chapters range from preparation of fluid specimens for analysis of cellular inflammatory responses to genotoxic insults to sensitive methods for proteomic analysis and aberrant DNA methylation patterns. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Molecular Toxicology Protocols, Second Edition addresses not only the needs of molecular biologists and toxicologists, but also those of individuals interested in applying molecular methods to clinical applications, such as geneticists, pathologists, biochemists, and epidemiologists
Member of
Cataloging source
GW5XE
Dewey number
615.9
Illustrations
illustrations
Index
index present
LC call number
RA1220.3
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
http://library.link/vocab/relatedWorkOrContributorName
  • Keohavong, Phouthone
  • Grant, Stephen G.
Series statement
Methods in molecular biology,
Series volume
v. 1105
http://library.link/vocab/subjectName
  • Molecular toxicology
  • DNA Mutational Analysis
  • DNA Adducts
  • Molecular Biology
  • Toxicology
Label
Molecular toxicology protocols, edited by Phouthone Keohavong, Stephen G. Grant, (electronic book)
Instantiates
Publication
Copyright
Antecedent source
unknown
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Color
multicolored
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
  • TaqMan Fluorogenic Detection System to Analyze Gene Transcription in Autopsy Material
  • 32P-Postlabeling Analysis of DNA Adducts
  • Modification of the 32P-Postlabeling Method to Detect a Single Adduct Species as a Single Spot
  • DNA Isolation and Sample Preparation for Quantification of Adduct Levels by Accelerator Mass Spectrometry
  • Analysis of DNA Strand Cleavage at Abasic Sites
  • Premature Chromosome Condensation in Human Resting Peripheral Blood Lymphocytes Without Mitogen Stimulation for Chromosome Aberration Analysis Using Specific Whole Chromosome DNA Hybridization Probes
  • Mutagen Sensitivity as Measured by Induced Chromatid Breakage as a Marker of Cancer Risk
  • Pulsed-Field Gel Electrophoresis Analysis of Multicellular DNA Double-Strand Break Damage and Repair
  • Detection of Pig-a Mutant Erythrocytes in the Peripheral Blood of Rats and Mice
  • The Blood-Based Glycophorin A (GPA) Human In Vivo Somatic Mutation Assay
  • Array-Based Immunoassays with Rolling-Circle Amplification Detection
  • Flow Cytometric Quantification of Mutant T Cells with Altered Expression of the T-Cell Receptor: Detecting Somatic Mutants in Humans and Mice
  • Analysis of In Vivo Mutation in the Hprt and Tk Genes of Mouse Lymphocytes
  • Analysis of Protein Changes Using Two-Dimensional Difference Gel Electrophoresis
  • Assessment of Pathological and Physiological Changes in Mouse Lung Through Bronchoalveolar Lavage
  • Analysis of Clinical and Biological Samples Using Microsphere-Based Multiplexing Luminex System
  • Detection of DNA Methylation by MeDIP and MBDCap Assays: An Overview of Techniques
  • Screening of DNA Methylation Changes by Methylation-Sensitive Random Amplified Polymorphic DNA-Polymerase Chain Reaction (MS-RAPD-PCR)
  • Strategies for Measurement of Biotransformation Enzyme Gene Expression
  • Genotyping Technologies: Application to Biotransformation Enzyme Genetic Polymorphism Screening
  • Gel-Based Nonradioactive Single-Strand Conformational Polymorphism and Mutation Detection: Limitations and Solutions
  • Detection and Characterization of Oncogene Mutations in Preneoplastic and Early Neoplastic Lesions
  • Quantitative PCR-Based Measurement of Nuclear and Mitochondrial DNA Damage and Repair in Mammalian Cells
  • The Sister Chromatid Exchange (SCE) Assay
  • The Gene Cluster Instability (GCI) Assay for Recombination
  • Measuring Recombination Proficiency in Mouse Embryonic Stem Cells
  • Microsatellite Instability: An Indirect Assay to Detect Defects in the Cellular Mismatch Repair Machinery
  • Unscheduled DNA Synthesis: The Clinical and Functional Assay for Global Genomic DNA Nucleotide Excision Repair
  • Analysis of Actively Transcribed DNA Repair Using a Transfection-Based System
  • An Immunoassay for Measuring Repair of UV Photoproducts
  • (Continued)
  • Analysis of Double-Strand Break Repair by Nonhomologous DNA End Joining in Cell-Free Extracts from Mammalian Cells
  • Bioenergetic Analysis of Intact Mammalian Cells Using the Seahorse XF24 Extracellular Flux Analyzer and a Luciferase ATP Assay
  • Quantification of Selective Phosphatidylserine Oxidation During Apoptosis
  • Quantitative Method of Measuring Phosphatidylserine Externalization During Apoptosis Using Electron Paramagnetic Resonance (EPR) Spectroscopy and Annexin-Conjugated Iron
  • Detection of Programmed Cell Death in Cells Exposed to Genotoxic Agents Using a Caspase Activation Assay
  • Quantifying In Vivo Somatic Mutations Using Transgenic Mouse Model Systems
  • The Human T-Cell Cloning Assay: Identifying Genotypes Susceptible to Drug Toxicity and Somatic Mutation
  • Molecular Analysis of Mutations in the Human HPRT Gene
  • Simultaneous Quantification of t(14;18) and HPRT Exon 2/3 Deletions in Human Lymphocytes
  • Mutation Screening of the TP53 Gene by Temporal Temperature Gel Electrophoresis (TTGE)
  • Detection of Point Mutations of K-ras Oncogene and p53 Tumor-Suppressor Gene in Sputum Samples
  • ACB-PCR Quantification of Somatic Oncomutation
Control code
SPR874025312
Dimensions
unknown
Edition
Second edition.
Extent
1 online resource (xv, 646 pages)
File format
unknown
Form of item
online
Isbn
9781627037389
Level of compression
unknown
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other control number
10.1007/978-1-62703-739-6
Other physical details
illustrations.
Quality assurance targets
not applicable
Reformatting quality
unknown
Reproduction note
Electronic resource.
Sound
unknown sound
Specific material designation
remote
Label
Molecular toxicology protocols, edited by Phouthone Keohavong, Stephen G. Grant, (electronic book)
Publication
Copyright
Antecedent source
unknown
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Color
multicolored
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
  • TaqMan Fluorogenic Detection System to Analyze Gene Transcription in Autopsy Material
  • 32P-Postlabeling Analysis of DNA Adducts
  • Modification of the 32P-Postlabeling Method to Detect a Single Adduct Species as a Single Spot
  • DNA Isolation and Sample Preparation for Quantification of Adduct Levels by Accelerator Mass Spectrometry
  • Analysis of DNA Strand Cleavage at Abasic Sites
  • Premature Chromosome Condensation in Human Resting Peripheral Blood Lymphocytes Without Mitogen Stimulation for Chromosome Aberration Analysis Using Specific Whole Chromosome DNA Hybridization Probes
  • Mutagen Sensitivity as Measured by Induced Chromatid Breakage as a Marker of Cancer Risk
  • Pulsed-Field Gel Electrophoresis Analysis of Multicellular DNA Double-Strand Break Damage and Repair
  • Detection of Pig-a Mutant Erythrocytes in the Peripheral Blood of Rats and Mice
  • The Blood-Based Glycophorin A (GPA) Human In Vivo Somatic Mutation Assay
  • Array-Based Immunoassays with Rolling-Circle Amplification Detection
  • Flow Cytometric Quantification of Mutant T Cells with Altered Expression of the T-Cell Receptor: Detecting Somatic Mutants in Humans and Mice
  • Analysis of In Vivo Mutation in the Hprt and Tk Genes of Mouse Lymphocytes
  • Analysis of Protein Changes Using Two-Dimensional Difference Gel Electrophoresis
  • Assessment of Pathological and Physiological Changes in Mouse Lung Through Bronchoalveolar Lavage
  • Analysis of Clinical and Biological Samples Using Microsphere-Based Multiplexing Luminex System
  • Detection of DNA Methylation by MeDIP and MBDCap Assays: An Overview of Techniques
  • Screening of DNA Methylation Changes by Methylation-Sensitive Random Amplified Polymorphic DNA-Polymerase Chain Reaction (MS-RAPD-PCR)
  • Strategies for Measurement of Biotransformation Enzyme Gene Expression
  • Genotyping Technologies: Application to Biotransformation Enzyme Genetic Polymorphism Screening
  • Gel-Based Nonradioactive Single-Strand Conformational Polymorphism and Mutation Detection: Limitations and Solutions
  • Detection and Characterization of Oncogene Mutations in Preneoplastic and Early Neoplastic Lesions
  • Quantitative PCR-Based Measurement of Nuclear and Mitochondrial DNA Damage and Repair in Mammalian Cells
  • The Sister Chromatid Exchange (SCE) Assay
  • The Gene Cluster Instability (GCI) Assay for Recombination
  • Measuring Recombination Proficiency in Mouse Embryonic Stem Cells
  • Microsatellite Instability: An Indirect Assay to Detect Defects in the Cellular Mismatch Repair Machinery
  • Unscheduled DNA Synthesis: The Clinical and Functional Assay for Global Genomic DNA Nucleotide Excision Repair
  • Analysis of Actively Transcribed DNA Repair Using a Transfection-Based System
  • An Immunoassay for Measuring Repair of UV Photoproducts
  • (Continued)
  • Analysis of Double-Strand Break Repair by Nonhomologous DNA End Joining in Cell-Free Extracts from Mammalian Cells
  • Bioenergetic Analysis of Intact Mammalian Cells Using the Seahorse XF24 Extracellular Flux Analyzer and a Luciferase ATP Assay
  • Quantification of Selective Phosphatidylserine Oxidation During Apoptosis
  • Quantitative Method of Measuring Phosphatidylserine Externalization During Apoptosis Using Electron Paramagnetic Resonance (EPR) Spectroscopy and Annexin-Conjugated Iron
  • Detection of Programmed Cell Death in Cells Exposed to Genotoxic Agents Using a Caspase Activation Assay
  • Quantifying In Vivo Somatic Mutations Using Transgenic Mouse Model Systems
  • The Human T-Cell Cloning Assay: Identifying Genotypes Susceptible to Drug Toxicity and Somatic Mutation
  • Molecular Analysis of Mutations in the Human HPRT Gene
  • Simultaneous Quantification of t(14;18) and HPRT Exon 2/3 Deletions in Human Lymphocytes
  • Mutation Screening of the TP53 Gene by Temporal Temperature Gel Electrophoresis (TTGE)
  • Detection of Point Mutations of K-ras Oncogene and p53 Tumor-Suppressor Gene in Sputum Samples
  • ACB-PCR Quantification of Somatic Oncomutation
Control code
SPR874025312
Dimensions
unknown
Edition
Second edition.
Extent
1 online resource (xv, 646 pages)
File format
unknown
Form of item
online
Isbn
9781627037389
Level of compression
unknown
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other control number
10.1007/978-1-62703-739-6
Other physical details
illustrations.
Quality assurance targets
not applicable
Reformatting quality
unknown
Reproduction note
Electronic resource.
Sound
unknown sound
Specific material designation
remote

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