Coverart for item
The Resource Mouse cell culture : methods and protocols, edited by Andrew Ward, David Tosh, (electronic book)

Mouse cell culture : methods and protocols, edited by Andrew Ward, David Tosh, (electronic book)

Label
Mouse cell culture : methods and protocols
Title
Mouse cell culture
Title remainder
methods and protocols
Statement of responsibility
edited by Andrew Ward, David Tosh
Contributor
Subject
Genre
Language
eng
Summary
Cultured cells have combined accessibility and the ability to expand a homogeneous cell population from a relatively limited source, thus opening up a wealth of possibilities for researchers. In Mouse Cell Culture: Methods and Protocols, expert researchers provide a number of methods for the culture of a wide range of specific cells and tissues isolated from the key genetic model of the fetal or adult mouse. Including protocols for the explant of fetal tissues and stem cells that allow developmental processes to be followed ex vivo as well as protocols for the culture of isolated cell types that allow for the study of relatively homogeneous cell populations, this volume brings together a selection of the most current methods in order to make them available in one convenient source. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Practical and authoritative, Mouse Cell Culture: Methods and Protocols serves as an immediately applicable springboard for the development of new tissue culture methods in order to advance the study and treatment of human disorders
Member of
Cataloging source
GW5XE
Dewey number
571.63819353
Illustrations
illustrations
Index
index present
LC call number
TP248.27.A53
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
http://library.link/vocab/relatedWorkOrContributorDate
1964-
http://library.link/vocab/relatedWorkOrContributorName
  • Ward, Andrew
  • Tosh, David
Series statement
  • Methods in molecular biology,
  • Springer protocols
Series volume
633
http://library.link/vocab/subjectName
  • Mice
  • Cell culture
  • Cell Culture Techniques
  • Mice
Label
Mouse cell culture : methods and protocols, edited by Andrew Ward, David Tosh, (electronic book)
Instantiates
Publication
Bibliography note
Includes bibliographical references and index
Color
multicolored
Contents
The culture of mouse embryonic stem cells and formation of embryoid bodies / Melany Jackson ... [et al.] -- Derivation of primary mouse embryonic fibroblast (PMEF) cultures / Alastair S. Garfield -- Embryonic skeletal muscle microexplant culture and isolation of skeletal muscle stem cells / Janet Smith and Deborah Merrick -- The embryonic kidney : isolation, organ culture, immunostaining, and RNA interference / Jamie A. Davies -- Explant culture of mouse embryonic whole lung, isolated epithelium, or mesenchyme under chemically defined conditions as a system to evaluate the molecular mechanism of branching morphogenesis and cellular differentiation / Pierre-Marie del Moral and David Warburton -- Isolation, culture, and characterisation of mouse embryonic oesophagus and intestine / Jonathan M. Quinlan, Wei-Yuan Yu, and David Tosh -- Isolation and culture of embryonic pancreas and liver / Zoë D. Burke ... [et al.] -- Isolation and culture of mouse satellite cells / Antonio Musarò and Laura Barberi -- Analysis of cardiac myocyte biology in transgenic mice : a protocol for preparation of neonatl mouse cardiac myocyte cultures / Nigel J. Brand ... [et al.] -- Short- and long-term cultivation of embryonic and neonatal murine keratinocytes / Reto Caldelari and Eliane J. Müller -- Isolation, culture, and analysis of mouse mammary epithelial cells / Matthew J. Smalley -- Isolation and culture of mouse pancreatic islets for ex vivo imaging studies with trappable or recombinant fluorescent probes / Magalie A. Ravier and Guy A. Rutter -- Isolation and culture of adult mouse hepatocytes / Wan-Chun Li, Kate L. Ralphs, and David Tosh -- Isolation and culture of mouse intestinal cells / Charles Frederick Campbell -- Derivation of primary choroid plexus epithelial cells from the mouse / Trevelyan R. Menheniott, Marika Charalambous, and Andrew Ward -- The preparation of primary cortical neuron cultures and a practical application using immunofluorescent cytochemistry / Carla Sciarretta and Liliana Minichiello -- Cell culture of primary cerebellar granule cells / Dana Kramer and Liliana Minichiello -- Isolation and generation of neurosphere cultures from embryonic and adult mouse brain / Henrik Ahlenius and Zaal Kokaia
Control code
SPR658898962
Dimensions
unknown
Extent
1 online resource (xi, 257 p.)
Form of item
online
Isbn
9781588297723
Other control number
10.1007/978-1-59745-019-5
Other physical details
ill. (some col.)
Reproduction note
Electronic resource.
Specific material designation
remote
Label
Mouse cell culture : methods and protocols, edited by Andrew Ward, David Tosh, (electronic book)
Publication
Bibliography note
Includes bibliographical references and index
Color
multicolored
Contents
The culture of mouse embryonic stem cells and formation of embryoid bodies / Melany Jackson ... [et al.] -- Derivation of primary mouse embryonic fibroblast (PMEF) cultures / Alastair S. Garfield -- Embryonic skeletal muscle microexplant culture and isolation of skeletal muscle stem cells / Janet Smith and Deborah Merrick -- The embryonic kidney : isolation, organ culture, immunostaining, and RNA interference / Jamie A. Davies -- Explant culture of mouse embryonic whole lung, isolated epithelium, or mesenchyme under chemically defined conditions as a system to evaluate the molecular mechanism of branching morphogenesis and cellular differentiation / Pierre-Marie del Moral and David Warburton -- Isolation, culture, and characterisation of mouse embryonic oesophagus and intestine / Jonathan M. Quinlan, Wei-Yuan Yu, and David Tosh -- Isolation and culture of embryonic pancreas and liver / Zoë D. Burke ... [et al.] -- Isolation and culture of mouse satellite cells / Antonio Musarò and Laura Barberi -- Analysis of cardiac myocyte biology in transgenic mice : a protocol for preparation of neonatl mouse cardiac myocyte cultures / Nigel J. Brand ... [et al.] -- Short- and long-term cultivation of embryonic and neonatal murine keratinocytes / Reto Caldelari and Eliane J. Müller -- Isolation, culture, and analysis of mouse mammary epithelial cells / Matthew J. Smalley -- Isolation and culture of mouse pancreatic islets for ex vivo imaging studies with trappable or recombinant fluorescent probes / Magalie A. Ravier and Guy A. Rutter -- Isolation and culture of adult mouse hepatocytes / Wan-Chun Li, Kate L. Ralphs, and David Tosh -- Isolation and culture of mouse intestinal cells / Charles Frederick Campbell -- Derivation of primary choroid plexus epithelial cells from the mouse / Trevelyan R. Menheniott, Marika Charalambous, and Andrew Ward -- The preparation of primary cortical neuron cultures and a practical application using immunofluorescent cytochemistry / Carla Sciarretta and Liliana Minichiello -- Cell culture of primary cerebellar granule cells / Dana Kramer and Liliana Minichiello -- Isolation and generation of neurosphere cultures from embryonic and adult mouse brain / Henrik Ahlenius and Zaal Kokaia
Control code
SPR658898962
Dimensions
unknown
Extent
1 online resource (xi, 257 p.)
Form of item
online
Isbn
9781588297723
Other control number
10.1007/978-1-59745-019-5
Other physical details
ill. (some col.)
Reproduction note
Electronic resource.
Specific material designation
remote

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