Coverart for item
The Resource Protein NMR techniques, edited by Alexander Shekhtman and David S. Burz, (electronic book)

Protein NMR techniques, edited by Alexander Shekhtman and David S. Burz, (electronic book)

Label
Protein NMR techniques
Title
Protein NMR techniques
Statement of responsibility
edited by Alexander Shekhtman and David S. Burz
Contributor
Editor
Subject
Genre
Language
eng
Summary
The field of protein NMR spectroscopy has rapidly expanded into new areas of biochemistry, molecular biology and cell biology research that were impossible to study as recently as ten years ago. This third edition of Protein NMR Techniques, expands upon the previous editions with current, detailed authoritative but down-to-earth descriptions of new methodologies. These include techniques for NMR sample preparation, solution and solid state NMR methodologies and data processing. Written in the highly successful Methods in Molecular Biology"!series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Protein NMR Techniques, Third Edition, seeks to aid scientists in understanding the latest innovations in the field of protein NMR
Member of
Cataloging source
GW5XE
Dewey number
616.07/548
Illustrations
illustrations
Index
index present
LC call number
QC762
LC item number
.P76 2012
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
http://library.link/vocab/relatedWorkOrContributorName
  • Shekhtman, Alexander
  • Burz, David S
Series statement
Methods in molecular biology
Series volume
831
http://library.link/vocab/subjectName
  • Nuclear magnetic resonance
  • Magnetic Resonance Spectroscopy
  • Protein
Label
Protein NMR techniques, edited by Alexander Shekhtman and David S. Burz, (electronic book)
Instantiates
Publication
Antecedent source
unknown
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Color
multicolored
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
A novel bacterial expression method with optimized parameters for very high yield production of triple-labeled proteins -- Isotopic labeling of heterologous proteins in the yeast Pichia pastoris and kluyveromyces lactis -- Isotope labeling in insect cells -- Isotope labeling in mammalian cells -- Cell-free protein production for NMR studies -- Cell-free membrane protein expression for solid-state NMR -- Expression and purification of Src-family kinases for solution NMR studies -- NMR studies of large protein systems -- Protein dynamics by (15)n nuclear magnetic relaxation -- Bacterial production and solution NMR studies of a viral membrane ion channel -- Preparation of the modular multi-domain protein RPA for study by NMR spectroscopy -- NMR studies of protein-RNA interactions -- Preparation and optimization of protein-DNA complexes suitable for detailed NMR studies -- NMR studies of protein-ligand interactions -- In-cell NMR spectroscopy in escherichia coli -- Deuterated peptides and proteins: Structure and dynamics studies by MAS solid-state NMR -- Solid-state NMR spectroscopy of protein complexes -- Synthesis, purification, and characterization of single helix membrane peptides and proteins for NMR spectroscopy -- Assignment of backbone resonances in a eukaryotic protein kinase -- ERK2 as a representative example -- Electrostatics of hydrogen exchange for analyzing protein flexibility -- Fast protein backbone NMR resonance assignment using the batch strategy -- Comprehensive automation for NMR structure determination of proteins -- Aria for solution and solid-state NMR -- Determining protein dynamics from (15)n relaxation data by using dynamics
Control code
SPR768761831
Dimensions
unknown
Edition
3rd ed.
Extent
1 online resource (xiv, 518 pages)
File format
unknown
Form of item
online
Isbn
9781617794797
Level of compression
unknown
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other physical details
illustrations.
Quality assurance targets
not applicable
Reformatting quality
unknown
Reproduction note
Electronic resource.
Sound
unknown sound
Specific material designation
remote
Label
Protein NMR techniques, edited by Alexander Shekhtman and David S. Burz, (electronic book)
Publication
Antecedent source
unknown
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Color
multicolored
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
A novel bacterial expression method with optimized parameters for very high yield production of triple-labeled proteins -- Isotopic labeling of heterologous proteins in the yeast Pichia pastoris and kluyveromyces lactis -- Isotope labeling in insect cells -- Isotope labeling in mammalian cells -- Cell-free protein production for NMR studies -- Cell-free membrane protein expression for solid-state NMR -- Expression and purification of Src-family kinases for solution NMR studies -- NMR studies of large protein systems -- Protein dynamics by (15)n nuclear magnetic relaxation -- Bacterial production and solution NMR studies of a viral membrane ion channel -- Preparation of the modular multi-domain protein RPA for study by NMR spectroscopy -- NMR studies of protein-RNA interactions -- Preparation and optimization of protein-DNA complexes suitable for detailed NMR studies -- NMR studies of protein-ligand interactions -- In-cell NMR spectroscopy in escherichia coli -- Deuterated peptides and proteins: Structure and dynamics studies by MAS solid-state NMR -- Solid-state NMR spectroscopy of protein complexes -- Synthesis, purification, and characterization of single helix membrane peptides and proteins for NMR spectroscopy -- Assignment of backbone resonances in a eukaryotic protein kinase -- ERK2 as a representative example -- Electrostatics of hydrogen exchange for analyzing protein flexibility -- Fast protein backbone NMR resonance assignment using the batch strategy -- Comprehensive automation for NMR structure determination of proteins -- Aria for solution and solid-state NMR -- Determining protein dynamics from (15)n relaxation data by using dynamics
Control code
SPR768761831
Dimensions
unknown
Edition
3rd ed.
Extent
1 online resource (xiv, 518 pages)
File format
unknown
Form of item
online
Isbn
9781617794797
Level of compression
unknown
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other physical details
illustrations.
Quality assurance targets
not applicable
Reformatting quality
unknown
Reproduction note
Electronic resource.
Sound
unknown sound
Specific material designation
remote

Library Locations

Processing Feedback ...