Coverart for item
The Resource RNA methodologies : laboratory guide for isolation and characterization, Robert E. Farrell Jr, (electronic book)

RNA methodologies : laboratory guide for isolation and characterization, Robert E. Farrell Jr, (electronic book)

Label
RNA methodologies : laboratory guide for isolation and characterization
Title
RNA methodologies
Title remainder
laboratory guide for isolation and characterization
Statement of responsibility
Robert E. Farrell Jr
Creator
Subject
Genre
Language
eng
Summary
This is the fourth edition of the successful laboratory guide which has translated the rich story of riboneucleic acid for over fifteen years. RNA Methodologies 4e presents the latest collection of tested laboratory protocols for the isolation and characterization of eukaryotic and prokaryotic RNA with greater emphasis on transcript profiling, including quantification issues and elucidation of alternative transcription start sites. Collectively the chapters work together providing analysis with clear take-home lessons to assist researchers to understand RNA and to optimize time at the bench. The abundant use of flow charts, tables and graphs are especially helpful in the planning and implementation phases of a project and facilitate learning. 30% new material in this edition includes the addition of RNA isolation protocols including RNA isolation from tissue, expansion of PCR optimization analysis and RNA interference sections, the introduction of a new chapter dealing with the molecular biology of plants, and an expanded glossary
Member of
Cataloging source
OPELS
http://library.link/vocab/creatorName
Farrell, Robert E.
Dewey number
572.88
Illustrations
illustrations
Index
index present
LC call number
QP623
LC item number
.F37 2010
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
http://library.link/vocab/subjectName
  • RNA
  • RNA
  • Molecular Biology
  • RNA
Label
RNA methodologies : laboratory guide for isolation and characterization, Robert E. Farrell Jr, (electronic book)
Instantiates
Publication
Note
Previous ed.: 2005
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Color
multicolored
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
CHAPTER 1: RNA and the Cellular Biochemistry Revisited -- CHAPTER 2: RNA Isolation Strategies -- CHAPTER 3: The Truth about Tissues -- CHAPTER 4: Going Green: RNA and the Molecular Biology of Plants (NEW) -- CHAPTER 5: Isolation of Polyadenylated RNA -- CHAPTER 6: Quality Control for RNA Preparations (EXPANDED) -- CHAPTER 7: Resilient Ribonucleases -- CHAPTER 8: Stringency: Conditions that Influence Nucleic Acid Structure (EXPANDED) -- CHAPTER 9: Electrophoresis of RNA (EXPANDED) -- CHAPTER 10: Photodocumentation and Image Analysis (EXPANDED) -- CHAPTER 11: Northern Analysis -- CHAPTER 12: Nucleic Acid Probe Technology -- CHAPTER 13: Practical Nucleic Acid Hybridization -- CHAPTER 14: Principles of Detection (EXPANDED) -- CHAPTER 15: Quantification of Specific mRNAs by Nuclease Protection -- CHAPTER 16: Analysis of Nuclear RNA (EXPANDED) -- CHAPTER 17: cDNA Synthesis (EXPANDED) -- CHAPTER 18: RT-PCR (NEW) -- CHAPTER 19: Quantitative PCR Techniques -- CHAPTER 20: Functional Genomics and Transcript Profiling (NEW) -- CHAPTER 21: High-Throughput Analysis of Gene Expression -- CHAPTER 22: Non-Array Methods for Global Analysis of Gene Expression (NEW) -- CHAPTER 23: RNA Interference: Targeted Gene Silencing (EXPANDED) -- CHAPTER 24: Genomes, Transcriptomes, Proteomes, and Bioinformatics -- CHAPTER 25: An RNA Paradigm
Control code
SCIDI528609521
Dimensions
unknown
Edition
4th ed.
Extent
1 online resource (xxiii, 717 p.)
Form of item
online
Isbn
9780080884950
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other physical details
ill.
Specific material designation
remote
Label
RNA methodologies : laboratory guide for isolation and characterization, Robert E. Farrell Jr, (electronic book)
Publication
Note
Previous ed.: 2005
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Color
multicolored
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
CHAPTER 1: RNA and the Cellular Biochemistry Revisited -- CHAPTER 2: RNA Isolation Strategies -- CHAPTER 3: The Truth about Tissues -- CHAPTER 4: Going Green: RNA and the Molecular Biology of Plants (NEW) -- CHAPTER 5: Isolation of Polyadenylated RNA -- CHAPTER 6: Quality Control for RNA Preparations (EXPANDED) -- CHAPTER 7: Resilient Ribonucleases -- CHAPTER 8: Stringency: Conditions that Influence Nucleic Acid Structure (EXPANDED) -- CHAPTER 9: Electrophoresis of RNA (EXPANDED) -- CHAPTER 10: Photodocumentation and Image Analysis (EXPANDED) -- CHAPTER 11: Northern Analysis -- CHAPTER 12: Nucleic Acid Probe Technology -- CHAPTER 13: Practical Nucleic Acid Hybridization -- CHAPTER 14: Principles of Detection (EXPANDED) -- CHAPTER 15: Quantification of Specific mRNAs by Nuclease Protection -- CHAPTER 16: Analysis of Nuclear RNA (EXPANDED) -- CHAPTER 17: cDNA Synthesis (EXPANDED) -- CHAPTER 18: RT-PCR (NEW) -- CHAPTER 19: Quantitative PCR Techniques -- CHAPTER 20: Functional Genomics and Transcript Profiling (NEW) -- CHAPTER 21: High-Throughput Analysis of Gene Expression -- CHAPTER 22: Non-Array Methods for Global Analysis of Gene Expression (NEW) -- CHAPTER 23: RNA Interference: Targeted Gene Silencing (EXPANDED) -- CHAPTER 24: Genomes, Transcriptomes, Proteomes, and Bioinformatics -- CHAPTER 25: An RNA Paradigm
Control code
SCIDI528609521
Dimensions
unknown
Edition
4th ed.
Extent
1 online resource (xxiii, 717 p.)
Form of item
online
Isbn
9780080884950
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other physical details
ill.
Specific material designation
remote

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