Coverart for item
The Resource RNAi : design and application, edited by Sailen Barik, (electronic book)

RNAi : design and application, edited by Sailen Barik, (electronic book)

Label
RNAi : design and application
Title
RNAi
Title remainder
design and application
Statement of responsibility
edited by Sailen Barik
Contributor
Subject
Genre
Language
eng
Summary
RNA interference (RNAi), in which RNA silences RNA, is the most recent discovery to revolutionize the study of biology. In RNAi: Design and Applications, leaders in the field contribute state-of-the-art, easy to follow methods and bench protocols designed for practical, everyday use of RNAi in biological research. Divided into two parts, this comprehensive volume covers fundamentals including designs of RNAi, biochemical assay protocols for the major components of RNAi, and study of potential off-target effects, followed by an extensive section covering various applications of RNAi in diverse model organisms and systems, from antiviral and anticancer applications to altering flower color in plants. Cutting edge and clearly written, RNAi: Design and Applications enables a researcher with standard molecular biological training to perform major RNAi-related experiments and contribute to this revolutionary, growing field
Member of
Cataloging source
STF
Dewey number
572.8/8
Illustrations
illustrations
Index
index present
LC call number
QP623.5.S63
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
http://library.link/vocab/relatedWorkOrContributorName
Barik, Sailen.
Series statement
Methods in molecular biology
Series volume
442
http://library.link/vocab/subjectName
  • Small interfering RNA
  • RNA Interference
  • RNA, Small Interefering
  • Gene Silencing
  • Genetic Techniques
Label
RNAi : design and application, edited by Sailen Barik, (electronic book)
Instantiates
Publication
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Color
mixed
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
Principles of dicer substrate (D-siRNA) design and function / Mohammed Amarzguioui and John J. Rossi -- Expression, purification, and analysis of recombinant Drosophila dicer-1 and dicer-2 enzymes / Xuecheng Ye and Qinghua Liu -- In vitro RNA cleavage assay for argonaute-family proteins / Keita Miyoshi [and others] -- Identifying siRNA-induced off-targets by microarray analysis / Emily Anderson [and others] -- Hydrodynamic delivery of siRNA in a mouse model of sepsis / Doreen E. Wesche-Soldato [and others] -- Nasal delivery of siRNA / Vira Bitko and Sailen Barik -- RNA interference as a genetic tool in trypanosomes / Vivian Bellofatto and Jennifer B. Palenchar -- Lentivirus-mediated RNA interference in mammalian neurons / Scott Q. Harper and Pedro Gonzalez-Alegre -- Silencing genes by RNA interference in the protozoan parasite Entamoeba histolytica / Carlos F. Solis and Nancy Guillén -- Use of RNAi in C. elegans / Tsuyoshi Ohkumo [and others] -- Application of siRNA against SARS in the rhesus macaque model / Qingquan Tang [and others] -- siRNA and shRNA as anticancer agents in a cervical cancer model / Wenyi Gu, Lisa Putral, and Nigel McMillan -- Identification and expression analysis of small RNAs during development / Toshiaki Watanabe, Hiroshi Imai, and Naojiro Minami -- Screening and identification of virus-encoded RNA silencing suppressors / Sumona Karjee, Mohammad Nurul Islam, and Sunil K. Mukherjee -- Application of RNA interference in functional genomics studies of a social insect / Michael E. Scharf, Xuguo Zhou, and Margaret A. Schwinghammer -- Temporal control of gene silencing by in ovo electroporation / Thomas Baeriswyl, Olivier Mauti, and Esther T. Stoeckli -- Altering flower color in transgenic plants by RNAi-mediated engineering of flavonoid biosynthetic pathway / Yoshikazu Tanaka, Noriko Nakamura, and Junichi Togami -- Transgenic RNA interference in mice / Pumin Zhang
Control code
SPR227399711
Dimensions
unknown
Extent
1 online resource (xiv, 270 pages)
Form of item
online
Isbn
9781597451918
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other physical details
illustrations (some color).
Reproduction note
Electronic resource.
Specific material designation
remote
Label
RNAi : design and application, edited by Sailen Barik, (electronic book)
Publication
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Color
mixed
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
Principles of dicer substrate (D-siRNA) design and function / Mohammed Amarzguioui and John J. Rossi -- Expression, purification, and analysis of recombinant Drosophila dicer-1 and dicer-2 enzymes / Xuecheng Ye and Qinghua Liu -- In vitro RNA cleavage assay for argonaute-family proteins / Keita Miyoshi [and others] -- Identifying siRNA-induced off-targets by microarray analysis / Emily Anderson [and others] -- Hydrodynamic delivery of siRNA in a mouse model of sepsis / Doreen E. Wesche-Soldato [and others] -- Nasal delivery of siRNA / Vira Bitko and Sailen Barik -- RNA interference as a genetic tool in trypanosomes / Vivian Bellofatto and Jennifer B. Palenchar -- Lentivirus-mediated RNA interference in mammalian neurons / Scott Q. Harper and Pedro Gonzalez-Alegre -- Silencing genes by RNA interference in the protozoan parasite Entamoeba histolytica / Carlos F. Solis and Nancy Guillén -- Use of RNAi in C. elegans / Tsuyoshi Ohkumo [and others] -- Application of siRNA against SARS in the rhesus macaque model / Qingquan Tang [and others] -- siRNA and shRNA as anticancer agents in a cervical cancer model / Wenyi Gu, Lisa Putral, and Nigel McMillan -- Identification and expression analysis of small RNAs during development / Toshiaki Watanabe, Hiroshi Imai, and Naojiro Minami -- Screening and identification of virus-encoded RNA silencing suppressors / Sumona Karjee, Mohammad Nurul Islam, and Sunil K. Mukherjee -- Application of RNA interference in functional genomics studies of a social insect / Michael E. Scharf, Xuguo Zhou, and Margaret A. Schwinghammer -- Temporal control of gene silencing by in ovo electroporation / Thomas Baeriswyl, Olivier Mauti, and Esther T. Stoeckli -- Altering flower color in transgenic plants by RNAi-mediated engineering of flavonoid biosynthetic pathway / Yoshikazu Tanaka, Noriko Nakamura, and Junichi Togami -- Transgenic RNA interference in mice / Pumin Zhang
Control code
SPR227399711
Dimensions
unknown
Extent
1 online resource (xiv, 270 pages)
Form of item
online
Isbn
9781597451918
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other physical details
illustrations (some color).
Reproduction note
Electronic resource.
Specific material designation
remote

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