Coverart for item
The Resource RNAi and plant gene function analysis : methods and protocols, edited by Hiroaki Kodama, Atsushi Komamine, (electronic book)

RNAi and plant gene function analysis : methods and protocols, edited by Hiroaki Kodama, Atsushi Komamine, (electronic book)

Label
RNAi and plant gene function analysis : methods and protocols
Title
RNAi and plant gene function analysis
Title remainder
methods and protocols
Statement of responsibility
edited by Hiroaki Kodama, Atsushi Komamine
Contributor
Subject
Genre
Language
eng
Summary
The use of RNAi technology is essential for most plant science researchers. As DNA sequence information increases, so the need for functional annotation of target genes also increases. Authoritative and accessible, RNAi and Plant Gene Function Analysis: Methods and Protocols seeks to serve as an ideal guide to scientists of all backgrounds looking to further their knowledge of this fundamental science. Written in the highly successful Methods in Molecular Biology series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. In addition to the functional annotation of unknown genes, RNAi technology has been applied to the genetic engineering of important plant metabolites including starches, oils, and storage proteins. RNAi has been used to engineer plants resistant to plant viruses and also to nematodes and insects. RNAi and Plant Gene Function Analysis: Methods and Protocols will provide both professionals and novices of plant biotechnology and breeding research with essential tips for designing experiments meant to explore plant gene function
Member of
Cataloging source
CUS
Dewey number
581.3/5
Illustrations
illustrations
Index
index present
LC call number
QK981
LC item number
.R53 2011
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
http://library.link/vocab/relatedWorkOrContributorName
  • Kodama, Hiroaki.
  • Komamine, Atsushi
Series statement
  • Springer protocols
  • Methods in molecular biology,
Series volume
744
http://library.link/vocab/subjectName
  • Plant genetics
  • Small interfering RNA
  • Plants
  • RNA Interference
Label
RNAi and plant gene function analysis : methods and protocols, edited by Hiroaki Kodama, Atsushi Komamine, (electronic book)
Instantiates
Publication
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Color
black and white
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
  • Gene function analysis by artificial microRNAs in Physcomitrella patens
  • Basel Khraiwesh [and others] -- Virus-induced gene silencing in ornamental plants
  • Cai-Zhong Jiang, Jen-Chih Chen, and Michael Reid
  • Local RNA silencing mediated by agroinfiltration
  • Jutta Maria Helm, Elena Dadami, and Kriton Kalantidis
  • Direct transfer of synthetic double-stranded RNA into protoplasts of Arabidopsis thaliana
  • Ha-il Jung, Zhiyang Zhai, and Olena K. Vatamaniuk
  • Detection of long and short double-stranded RNAs
  • Toshiyuki Fukuhara [and others]
  • Quantitative stem-loop RT-PCR for detection of microRNAs
  • Overview of plant RNAi
  • Erika Varkonyi-Gasic and Roger P. Hellens
  • Large-scale sequencing of plant small RNAs
  • William P. Donovan, Yuanji Zhang, and Miya D. Howell
  • Computational prediction of plant miRNA targets
  • Ying-Hsuan Sun [and others]
  • Bisulfite sequencing for cytosine-methylation analysis in plants
  • Nazmul Haque and Masamichi Nishiguchi
  • Using nuclear run-on transcription assays in RNAi studies
  • Basel Khraiwesh
  • Proteomic analysis of RNA interference induced knockdown plant
  • Yuichiro Watanabe
  • Sang Yeol Lee and Kyun Oh Lee
  • Comparative analysis of phosphoprotein expression using 2D-DIGE
  • Tomoya Asano and Takumi Nishiuchi
  • Caveat of RNAi in plants : the off-target effect
  • Muthappa Senthil-Kumar and Kirankumar S. Mysore
  • Plant gateway vectors for RNAi as a tool for functional genomic studies
  • Toshiya Muranaka
  • Heat-inducible RNAi for gene functional analysis in plants
  • Frederic Masclaux and Jean-Philippe Galaud
Control code
SPR722926868
Dimensions
unknown
Extent
1 online resource (x, 244 pages)
Form of item
online
Isbn
9781617791239
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other physical details
illustrations.
Reproduction note
Electronic resource.
Sound
unknown sound
Specific material designation
remote
Label
RNAi and plant gene function analysis : methods and protocols, edited by Hiroaki Kodama, Atsushi Komamine, (electronic book)
Publication
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Color
black and white
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
  • Gene function analysis by artificial microRNAs in Physcomitrella patens
  • Basel Khraiwesh [and others] -- Virus-induced gene silencing in ornamental plants
  • Cai-Zhong Jiang, Jen-Chih Chen, and Michael Reid
  • Local RNA silencing mediated by agroinfiltration
  • Jutta Maria Helm, Elena Dadami, and Kriton Kalantidis
  • Direct transfer of synthetic double-stranded RNA into protoplasts of Arabidopsis thaliana
  • Ha-il Jung, Zhiyang Zhai, and Olena K. Vatamaniuk
  • Detection of long and short double-stranded RNAs
  • Toshiyuki Fukuhara [and others]
  • Quantitative stem-loop RT-PCR for detection of microRNAs
  • Overview of plant RNAi
  • Erika Varkonyi-Gasic and Roger P. Hellens
  • Large-scale sequencing of plant small RNAs
  • William P. Donovan, Yuanji Zhang, and Miya D. Howell
  • Computational prediction of plant miRNA targets
  • Ying-Hsuan Sun [and others]
  • Bisulfite sequencing for cytosine-methylation analysis in plants
  • Nazmul Haque and Masamichi Nishiguchi
  • Using nuclear run-on transcription assays in RNAi studies
  • Basel Khraiwesh
  • Proteomic analysis of RNA interference induced knockdown plant
  • Yuichiro Watanabe
  • Sang Yeol Lee and Kyun Oh Lee
  • Comparative analysis of phosphoprotein expression using 2D-DIGE
  • Tomoya Asano and Takumi Nishiuchi
  • Caveat of RNAi in plants : the off-target effect
  • Muthappa Senthil-Kumar and Kirankumar S. Mysore
  • Plant gateway vectors for RNAi as a tool for functional genomic studies
  • Toshiya Muranaka
  • Heat-inducible RNAi for gene functional analysis in plants
  • Frederic Masclaux and Jean-Philippe Galaud
Control code
SPR722926868
Dimensions
unknown
Extent
1 online resource (x, 244 pages)
Form of item
online
Isbn
9781617791239
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other physical details
illustrations.
Reproduction note
Electronic resource.
Sound
unknown sound
Specific material designation
remote

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