Coverart for item
The Resource Retinal development : methods and protocols, edited by Shu-Zhen Wang, (electronic book)

Retinal development : methods and protocols, edited by Shu-Zhen Wang, (electronic book)

Label
Retinal development : methods and protocols
Title
Retinal development
Title remainder
methods and protocols
Statement of responsibility
edited by Shu-Zhen Wang
Contributor
Editor
Subject
Genre
Language
eng
Summary
In recent years, there have been major advances in the concepts and methodologies used in the study of retinal development at both cellular and molecular levels. These advanced methodologies have allowed and will continue to allow researchers to gain new insights into the molecular mechanisms underlying retinal development. In Retinal Development: Methods and Protocols, expert researchers in the field detail many of the protocols used for a wide range of experiments. These include protocols and techniques for manipulating gene expression in vivo, tracing cell fates with modernized classic blastomere manipulation in Xenopus and with Cre-based technique in mouse and in zebrafish, retinal regeneration and stem cell-based replacement, and ERG (function) recording and non-invasive imaging. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Retinal Development: Methods and Protocols provides methodologies crucial to the success of increasingly more complex and often challenging investigations in the fields of retinal development and other biological and biomedical research
Member of
Cataloging source
GW5XE
Dewey number
612.8/43
Illustrations
illustrations
Index
index present
LC call number
QP479
LC item number
.R48 2012
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
http://library.link/vocab/relatedWorkOrContributorName
Wang, Shu-Zhen
Series statement
Methods in molecular biology,
Series volume
884
http://library.link/vocab/subjectName
  • Retina
  • Retina
Label
Retinal development : methods and protocols, edited by Shu-Zhen Wang, (electronic book)
Instantiates
Publication
Antecedent source
unknown
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Color
multicolored
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
  • In ovo eye electroporation
  • Teri L. Belecky-Adams, Scott R. Hudson, and Sarika Tiwari
  • Targeted microinjection of synthetic mRNAs to alter retina gene expression in Xenopus embryos
  • Sally A. Moody
  • Testing retina fate commitment in Xenopus by blastomere deletion, transplantation, and explant culture
  • Sally A. Moody
  • Application of Cre-loxP recombination for lineage tracing of adult zebrafish retinal stem cells
  • Rajesh Ramachandran, Aaron Reifler, Jin Wan, and Daniel Goldman
  • Fate tracing of neurogenin2-expressing cells in the mouse retina using CreER(TM) : LacZ
  • Wenxin Ma and Shu-Zhen Wang
  • Conditional control of gene expression in the mouse retina
  • In vitro explant culture and related protocols for the study of mouse retinal development
  • Kangxin Jin and Mengqing Xiang
  • In vitro biochemical assays to monitor rhodopsin function
  • Joshua Sammons and Alecia K. Gross
  • Transfection of primary embryonic chicken retinal cells using cationic lipid
  • Yi-Wen Hsieh and Xian-Jie Yang
  • Production of high-titer RCAS retrovirus
  • Run-Tao Yan and Shu-Zhen Wang
  • Chick retinal pigment epithelium transdifferentiation assay for proneural activities
  • Shu-Zhen Wang and Run-Tao Yan
  • Qian Ding and Lin Gan
  • Studying the generation of regenerated retinal neuron from muller glia in the mouse eye.
  • Mike O. Karl and Thomas A. Reh
  • Production and transplantation of retinal cells from human and mouse embryonic stem cells
  • Anna La Torre, Deepak A. Lamba, Anu Jayabalu, and Thomas A. Reh
  • Light-induced photoreceptor degeneration in the Retina of the zebrafish
  • Scott Taylor, Jing Chen, Jing Luo, and Peter Hitchcock
  • Microarray-based gene profiling analysis of muller glia-derived retinal stem cells in light-damaged retinas from adult zebrafish
  • Zhao Qin and Pamela A. Raymond
  • Measuring rodent electroretinograms to assess retinal function
  • Molly E. Clark and Timothy W. Kraft
  • Generation of transgenic Xenopus using restriction enzyme-mediated integration
  • Functional imaging of retinal photoreceptors and inner neurons using stimulus-evoked intrinsic optical signals
  • Xin-Cheng Yao and Yi-Chao Li
  • Use of laser capture microdissection for analysis of retinal mRNA/miRNA Expression and DNA Methylation
  • Laszlo Hackler Jr, Tomohiro Masuda, Verity F. Oliver, Shannath L. Merbs, and Donald J. Zack
  • Revealing looping organization of mammalian photoreceptor genes using chromosome conformation capture (3C) assays
  • Guang-Hua Peng and Shiming Chen
  • Retinal transcriptome profiling by directional next-generation sequencing using 100 ng of total RNA
  • Matthew J. Brooks, Harsha Karur Rajasimha, and Anand Swaroop
  • Exome sequencing : capture and sequencing of all human coding regions for disease gene discovery
  • Rinki Ratna Priya, Harsha Karur Rajasimha, Matthew J. Brooks, and Anand Swaroop
  • Mohammad Haeri and Barry E. Knox
  • Determination of posttranslational modifications of photoreceptor differentiation factor NRL : focus on SUMOylation
  • Jerome E. Roger, Jacob Nellissery, and Anand Swaroop
  • In vivo functional analysis of transcription factor : response element interaction using transgenic Xenopus laevis
  • Heithem M. El-Hodiri, Yi Pan, and Lisa E. Kelly
  • Subretinal delivery and electroporation in pigmented and nonpigmented adult mouse eyes
  • John M. Nickerson, Penny Goodman, Micah A. Chrenek, Christiana J. Bernal, Lennart Berglin, T. Michael Redmond, and Jeffrey H. Boatright
Control code
SPR795994038
Dimensions
unknown
Extent
1 online resource (xiv, 367 pages)
File format
unknown
Form of item
online
Isbn
9781617798481
Level of compression
unknown
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other control number
10.1007/978-1-61779-848-1
Other physical details
illustrations (some color).
Quality assurance targets
not applicable
Reformatting quality
unknown
Reproduction note
Electronic resource.
Sound
unknown sound
Specific material designation
remote
Label
Retinal development : methods and protocols, edited by Shu-Zhen Wang, (electronic book)
Publication
Antecedent source
unknown
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Color
multicolored
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
  • In ovo eye electroporation
  • Teri L. Belecky-Adams, Scott R. Hudson, and Sarika Tiwari
  • Targeted microinjection of synthetic mRNAs to alter retina gene expression in Xenopus embryos
  • Sally A. Moody
  • Testing retina fate commitment in Xenopus by blastomere deletion, transplantation, and explant culture
  • Sally A. Moody
  • Application of Cre-loxP recombination for lineage tracing of adult zebrafish retinal stem cells
  • Rajesh Ramachandran, Aaron Reifler, Jin Wan, and Daniel Goldman
  • Fate tracing of neurogenin2-expressing cells in the mouse retina using CreER(TM) : LacZ
  • Wenxin Ma and Shu-Zhen Wang
  • Conditional control of gene expression in the mouse retina
  • In vitro explant culture and related protocols for the study of mouse retinal development
  • Kangxin Jin and Mengqing Xiang
  • In vitro biochemical assays to monitor rhodopsin function
  • Joshua Sammons and Alecia K. Gross
  • Transfection of primary embryonic chicken retinal cells using cationic lipid
  • Yi-Wen Hsieh and Xian-Jie Yang
  • Production of high-titer RCAS retrovirus
  • Run-Tao Yan and Shu-Zhen Wang
  • Chick retinal pigment epithelium transdifferentiation assay for proneural activities
  • Shu-Zhen Wang and Run-Tao Yan
  • Qian Ding and Lin Gan
  • Studying the generation of regenerated retinal neuron from muller glia in the mouse eye.
  • Mike O. Karl and Thomas A. Reh
  • Production and transplantation of retinal cells from human and mouse embryonic stem cells
  • Anna La Torre, Deepak A. Lamba, Anu Jayabalu, and Thomas A. Reh
  • Light-induced photoreceptor degeneration in the Retina of the zebrafish
  • Scott Taylor, Jing Chen, Jing Luo, and Peter Hitchcock
  • Microarray-based gene profiling analysis of muller glia-derived retinal stem cells in light-damaged retinas from adult zebrafish
  • Zhao Qin and Pamela A. Raymond
  • Measuring rodent electroretinograms to assess retinal function
  • Molly E. Clark and Timothy W. Kraft
  • Generation of transgenic Xenopus using restriction enzyme-mediated integration
  • Functional imaging of retinal photoreceptors and inner neurons using stimulus-evoked intrinsic optical signals
  • Xin-Cheng Yao and Yi-Chao Li
  • Use of laser capture microdissection for analysis of retinal mRNA/miRNA Expression and DNA Methylation
  • Laszlo Hackler Jr, Tomohiro Masuda, Verity F. Oliver, Shannath L. Merbs, and Donald J. Zack
  • Revealing looping organization of mammalian photoreceptor genes using chromosome conformation capture (3C) assays
  • Guang-Hua Peng and Shiming Chen
  • Retinal transcriptome profiling by directional next-generation sequencing using 100 ng of total RNA
  • Matthew J. Brooks, Harsha Karur Rajasimha, and Anand Swaroop
  • Exome sequencing : capture and sequencing of all human coding regions for disease gene discovery
  • Rinki Ratna Priya, Harsha Karur Rajasimha, Matthew J. Brooks, and Anand Swaroop
  • Mohammad Haeri and Barry E. Knox
  • Determination of posttranslational modifications of photoreceptor differentiation factor NRL : focus on SUMOylation
  • Jerome E. Roger, Jacob Nellissery, and Anand Swaroop
  • In vivo functional analysis of transcription factor : response element interaction using transgenic Xenopus laevis
  • Heithem M. El-Hodiri, Yi Pan, and Lisa E. Kelly
  • Subretinal delivery and electroporation in pigmented and nonpigmented adult mouse eyes
  • John M. Nickerson, Penny Goodman, Micah A. Chrenek, Christiana J. Bernal, Lennart Berglin, T. Michael Redmond, and Jeffrey H. Boatright
Control code
SPR795994038
Dimensions
unknown
Extent
1 online resource (xiv, 367 pages)
File format
unknown
Form of item
online
Isbn
9781617798481
Level of compression
unknown
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other control number
10.1007/978-1-61779-848-1
Other physical details
illustrations (some color).
Quality assurance targets
not applicable
Reformatting quality
unknown
Reproduction note
Electronic resource.
Sound
unknown sound
Specific material designation
remote

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