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The Resource Single nucleotide polymorphisms : methods and protocols, edited by Pui-Yan Kwok, (electronic book)

Single nucleotide polymorphisms : methods and protocols, edited by Pui-Yan Kwok, (electronic book)

Label
Single nucleotide polymorphisms : methods and protocols
Title
Single nucleotide polymorphisms
Title remainder
methods and protocols
Statement of responsibility
edited by Pui-Yan Kwok
Contributor
Subject
Genre
Language
eng
Summary
Single nucleotide polymorphisms (SNPs) have become the markers of choice in elucidating the relationship between DNA sequence variation and susceptibility to disease and have clearly become the focus of the next phase of the human genome project. In Single Nucleotide Polymorphisms: Methods and Protocols, Pui-Yan Kwok, MD, PhD, has assembled a collection of robust techniques for the difficult process of SNP discovery and genotyping. These cutting-edge protocols for mutation/SNP detection utilize denaturing high-performance liquid chromatography (dHPLC), single-strand conformation polymorphism (SSCP), conformation-sensitive gel electrophoresis (CSGE), chemical cleavage, and direct sequencing. Equally powerful and up-to-date methods are given for genotyping SNPs, including molecular beacons, the Taqman assay, single-base extension approaches, pyrosequencing, ligation, the Invader assay, and primer extension with mass spectrometry detection. Described in step-by-step detail by their inventors, each method provides extensive notes on the technical steps critical for experimental success, time-saving techniques, and tips on avoiding pitfalls. Comprehensive and authoritative, Single Nucleotide Polymorphisms: Methods and Protocols provides in a readily reproducible format all the major SNP discovery and genotyping techniques in use today, whether for using DNA diagnostics to identify a pathogen, for studying the genetic basis of human disease, or for molecular breeding programs in agriculture
Member of
Additional physical form
Also issued in print.
Cataloging source
COO
Dewey number
611/.01816
Illustrations
illustrations
Index
index present
LC call number
QH447.6
LC item number
.S565 2003
Literary form
non fiction
NAL call number
QH506.M45
NAL item number
v.212
Nature of contents
  • dictionaries
  • bibliography
http://library.link/vocab/relatedWorkOrContributorDate
1956-
http://library.link/vocab/relatedWorkOrContributorName
Kwok, Pui-Yan
Series statement
Methods in molecular biology
Series volume
212
http://library.link/vocab/subjectName
  • Chromosome polymorphism
  • Human genetics
  • Genetic markers
  • MEDICAL
  • Electronic books
Label
Single nucleotide polymorphisms : methods and protocols, edited by Pui-Yan Kwok, (electronic book)
Instantiates
Publication
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
SNPs: why do we care? / Lisa D. Brooks -- Denaturing high-performance liquid chromatography / Andreas Premstaller and Peter J. Oefner -- SNP detection and allele frequency determination by SSCP / Tomoko Tahira ... [et al.] -- Conformation-sensitive gel electrophoresis / Arupa Ganguly -- Detection of mutations in DNA by solid-phase chemical cleavage method: a simplified assay / Chinh T. Bui ... [et al.] -- SNP discovery by direct DNA sequencing / Pui-Yan Kwok and Shenghui Duan -- Computational SNP discovery in DNA sequence data / Gabor T. Marth -- Genotyping SNPs with molecular beacons / Salvatore A.E. Marras, Fred Russell Kramer, and Sanjay Tyagi -- SNP genotyping by the 51-nuclease reaction / Kenneth J. Livak -- Genotyping SNPs by minisequencing primer extension using oligonucliotide microarrays / Katarina Lindroos, Ulrika Liljedahl, and Ann-Christine Syvänen -- Quantitative analysis of SNPs in pooled DNA samples by solid-phase minisequencing / Charlotta Olsson, Ulrika Liljedahl, and Ann-Christine Syvänen -- Homogeneous primer extension assay with fluorescence polarization detection / Tony M. Hsu and Pui-Yan Kwok -- Pyrosequencing for SNP genotyping / Mostafa Ronaghi -- Homogeneous allele-specific PCR in SNP genotyping / Søren Germer and Russell Higuchi -- Oligonucleotide ligation assay / Jonas Jarvius, Mats Nilsson, and Ulf Landegren -- Invader assay for SNP genotyping / Victor Lyamichev and Bruce Neri -- MALDI-TOF mass spectrometry-based SNP genotyping / Niels Storm ... [et al.]
Control code
SPR52440528
Dimensions
unknown
Extent
1 online resource (xi, 269 p.)
Form of item
online
Isbn
9781592593279
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other physical details
ill.
Specific material designation
remote
Label
Single nucleotide polymorphisms : methods and protocols, edited by Pui-Yan Kwok, (electronic book)
Publication
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
SNPs: why do we care? / Lisa D. Brooks -- Denaturing high-performance liquid chromatography / Andreas Premstaller and Peter J. Oefner -- SNP detection and allele frequency determination by SSCP / Tomoko Tahira ... [et al.] -- Conformation-sensitive gel electrophoresis / Arupa Ganguly -- Detection of mutations in DNA by solid-phase chemical cleavage method: a simplified assay / Chinh T. Bui ... [et al.] -- SNP discovery by direct DNA sequencing / Pui-Yan Kwok and Shenghui Duan -- Computational SNP discovery in DNA sequence data / Gabor T. Marth -- Genotyping SNPs with molecular beacons / Salvatore A.E. Marras, Fred Russell Kramer, and Sanjay Tyagi -- SNP genotyping by the 51-nuclease reaction / Kenneth J. Livak -- Genotyping SNPs by minisequencing primer extension using oligonucliotide microarrays / Katarina Lindroos, Ulrika Liljedahl, and Ann-Christine Syvänen -- Quantitative analysis of SNPs in pooled DNA samples by solid-phase minisequencing / Charlotta Olsson, Ulrika Liljedahl, and Ann-Christine Syvänen -- Homogeneous primer extension assay with fluorescence polarization detection / Tony M. Hsu and Pui-Yan Kwok -- Pyrosequencing for SNP genotyping / Mostafa Ronaghi -- Homogeneous allele-specific PCR in SNP genotyping / Søren Germer and Russell Higuchi -- Oligonucleotide ligation assay / Jonas Jarvius, Mats Nilsson, and Ulf Landegren -- Invader assay for SNP genotyping / Victor Lyamichev and Bruce Neri -- MALDI-TOF mass spectrometry-based SNP genotyping / Niels Storm ... [et al.]
Control code
SPR52440528
Dimensions
unknown
Extent
1 online resource (xi, 269 p.)
Form of item
online
Isbn
9781592593279
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other physical details
ill.
Specific material designation
remote

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