Enzyme engineering : methods and protocols

Resource Information

The work Enzyme engineering : methods and protocols represents a distinct intellectual or artistic creation found in University of Liverpool. This resource is a combination of several types including: Work, Language Material, Books.

Label

Enzyme engineering : methods and protocols

Title remainder

methods and protocols

Statement of responsibility

edited by James C. Samuelson

Contributor

• Samuelson, James C., 1968-
• Samuelson, James C., 1968-

Subject

• Biomedical Engineering -- Laboratory Manuals
• Enzymes -- Biotechnology
• Enzymes -- Industrial applications
• Enzymes -- Laboratory Manuals
• Protein engineering

Genre

• Laboratory Manuals

Language

eng

Summary

Whether the pursuit is commercially motivated or purely academic, engineering a novel biological catalyst is an enticing challenge. High-resolution protein structure analysis allows for rational alteration of enzyme function, yet many useful enzyme variants are the product of well-designed selection schemes or screening strategies. Enzyme Engineering: Methods and Protocols provides guidance to investigators wishing to create enzyme variants with desired properties. This detailed volume covers such topics as a simple method for generating site-specific mutations within bacterial chromosomes. It also highlights the engineering of two difference types of rare-cutting endonucleases that show great potential in gene therapy applications: The newest development is the emergence of TAL effector nucleases or TALENs. Chapters describe newly developed technologies in sufficient detail so that each method can be practiced in a standard molecular biology laboratory. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible Enzyme Engineering: Methods and Protocols will be valuable for scientists with a budding interest in protein engineering as well as veterans looking for new approaches to apply in established discovery programs

Member of

• Online access with purchase: Springer
• Methods in molecular biology (Clifton, N.J.), v. 978.

Cataloging source

SCB

Dewey number

572/.7

Illustrations

illustrations

Index

index present

LC call number

QP601

LC item number

.E51575 2013

Literary form

non fiction

Nature of contents

• dictionaries
• bibliography

Series statement

Methods in molecular biology,

Series volume

v. 978