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The Resource Practical methods in cardiovascular research, Stefan Dhein, Friedrich Wilhelm Mohr, Mario Delmar [editors], (electronic book)

Practical methods in cardiovascular research, Stefan Dhein, Friedrich Wilhelm Mohr, Mario Delmar [editors], (electronic book)

Label
Practical methods in cardiovascular research
Title
Practical methods in cardiovascular research
Statement of responsibility
Stefan Dhein, Friedrich Wilhelm Mohr, Mario Delmar [editors]
Contributor
Subject
Language
eng
Summary
"Cardiovascular disease is among the most common causes of death in Western industrialized countries. Although a number of diagnostic and therapeutic possibilities exist today, many problems remain unsolved. This implies the urgent need for future cardiovascular research. Practical Methods in Cardiovascular Research gives an overview of a number of available techniques most often used in cardiovascular sciences, especially for investigators coming from other fields of research." "Practical Methods in Cardiovascular Research is a book that will be welcomed by workers in all fields of cardiovascular science, cardiovascular researchers, cardiologists, and physiologists alike as an indispensable laboratory companion."--Jacket
Cataloging source
DE-He213
Characteristic
online system or service
Image bit depth
0
http://library.link/vocab/relatedWorkOrContributorName
  • Dhein, Stefan
  • Mohr, Friedrich Wilhelm
  • Delmar, Mario
http://library.link/vocab/subjectName
  • Cardiovascular systems
  • Cardiovascular diseases
  • Chemistry, analytical
  • Clinical laboratory techniques
Label
Practical methods in cardiovascular research, Stefan Dhein, Friedrich Wilhelm Mohr, Mario Delmar [editors], (electronic book)
Instantiates
Publication
Antecedent source
mixed
Bibliography note
Includes bibliographical references and index
Color
not applicable
Contents
Cover -- Table of Contents -- In-Vivo Techniques -- 1 Models of Cardiovascular Diseases -- 1.1 Anaesthesia (Inhalative or i.v. Narcosis) of Laboratory Animals -- 1.2 Extracorporeal Circulation -- 1.3 Open-chest Models of Acute Myocardial Ischemia and Reperfusion -- 1.4 Models of Chronic Ischemia and Infarction -- 1.5 Models on Left Ventricular Hypertrophy (LVH) -- 1.6 Experimental Models of Heart Failure -- 1.7 In-Vivo Models of Arrhythmias: a Canine Model of Sudden Cardiac Death -- 1.8 In-Vivo Models of Atrial Fibrillation -- In-Vitro Techniques -- 2 Isolated Organs -- 2.1 The Langendorff Heart -- 2.2 The Working Heart -- 2.3 Isolated Papillary Muscles -- 2.4 Isolated Vessels -- 3 Electrophysiological Techniques -- 3.1 Optical Techniques for the Recording of Action Potentials -- 3.2 Recording Action Potentials Using Voltage-Sensitive Dyes -- 3.3 Epicardial Mapping in Isolated Hearts ... Use in Safety Pharmacology, Analysis of Torsade de Pointes Arrhythmia and Ischemia-Reperfusion-Related Arrhythmia -- 3.4 Voltage-Clamp and Patch-Clamp Techniques -- 3.5 L-Type Calcium Channel Recording -- 3.6 Recording Cardiac Potassium Currents with the Whole-Cell Voltage Clamp Technique -- 3.7 Recording the Pacemaker Current If -- 3.8 Recording Gap Junction Currents -- 3.9 Recording Monophasic Action Potentials -- 3.10 Heart on a Chip ... Extracellular Multielectrode Recordings from Cardiac Myocytes in Vitro -- 4 Histological Techniques -- 4.1 Immunohistochemistry for Structural and Functional Analysis in Cardiovascular Research -- 4.2 Classical Histological Staining Procedures in Cardiovascular Research -- 4.3 Practical Use of in situ Hybridisation and RT in situ PCR in Cardiovascular Research -- 4.4 Practical Use of Transmission Electron Microscopy for Analysis of Structures and Molecules in Cardiovascular Research -- 4.5 DAF Technique for Real-Time NO Imaging in the Human Myocardium -- 5 Cell Culture Techniques -- 5.1 Isolation and Culture of Adult Ventricular Cardiomyocytes -- 5.2 Culture of Neonatal Cardiomyocytes -- 5.3 Culture of Embryoid Bodies -- 5.4 Culturing and Differentiation of Embryonic and Adult Stem Cells for Heart Research and Transplantation Therapy -- 5.5 Preparation of Endothelial Cells from Micro- and Macrovascular Origin -- 5.6 In Vitro Cultivation of Vascular Smooth Muscle Cells -- 5.7 Engineering Heart Tissue for In Vitro and In Vivo Studies -- 6 Biochemical and Analytical Techniques -- 6.1 High-Performance Liquid Chromatography (HPLC) -- 6.2 Protein Biochemistry -- 6.3 Receptor and Binding Studies -- 6.4 Recording and Analysing Concentration-Response Curves -- 6.5 Measurement of NO and Endothelial Function in Cardiovascular Research -- 6.6 Biomechanical Stimulation of Vascular Cells In Vitro -- 6.7 Measurement of Function and Regulation of Adrenergic Receptors -- 6.8 Measurement of Function and Regulation of Muscarinic Acetylcholine Receptors -- 6.9 Cytometry in Cardiovascular Research -- 7 Molecular Biology Techniques -- 7.1 Real-Time PCR Analysis of Cardiac Samples -- 7.2 Use of cDNA Arrays to Explore Gene Expression in Genetically Manipulated Mice and Cell Lines -- 7.3 Using Antibody Arrays to Detect Protein-Protein Interactions -- 7.4 Surface Plasmon Resonance as a Method to Study the Kinetics and Amplitude of Protein-Protein Binding -- 7.5 How to Solve a Protein Structure by
Dimensions
unknown
File format
multiple file formats
Isbn
9783540265740
Level of compression
uncompressed
Quality assurance targets
absent
Reformatting quality
access
Specific material designation
remote
Label
Practical methods in cardiovascular research, Stefan Dhein, Friedrich Wilhelm Mohr, Mario Delmar [editors], (electronic book)
Publication
Antecedent source
mixed
Bibliography note
Includes bibliographical references and index
Color
not applicable
Contents
Cover -- Table of Contents -- In-Vivo Techniques -- 1 Models of Cardiovascular Diseases -- 1.1 Anaesthesia (Inhalative or i.v. Narcosis) of Laboratory Animals -- 1.2 Extracorporeal Circulation -- 1.3 Open-chest Models of Acute Myocardial Ischemia and Reperfusion -- 1.4 Models of Chronic Ischemia and Infarction -- 1.5 Models on Left Ventricular Hypertrophy (LVH) -- 1.6 Experimental Models of Heart Failure -- 1.7 In-Vivo Models of Arrhythmias: a Canine Model of Sudden Cardiac Death -- 1.8 In-Vivo Models of Atrial Fibrillation -- In-Vitro Techniques -- 2 Isolated Organs -- 2.1 The Langendorff Heart -- 2.2 The Working Heart -- 2.3 Isolated Papillary Muscles -- 2.4 Isolated Vessels -- 3 Electrophysiological Techniques -- 3.1 Optical Techniques for the Recording of Action Potentials -- 3.2 Recording Action Potentials Using Voltage-Sensitive Dyes -- 3.3 Epicardial Mapping in Isolated Hearts ... Use in Safety Pharmacology, Analysis of Torsade de Pointes Arrhythmia and Ischemia-Reperfusion-Related Arrhythmia -- 3.4 Voltage-Clamp and Patch-Clamp Techniques -- 3.5 L-Type Calcium Channel Recording -- 3.6 Recording Cardiac Potassium Currents with the Whole-Cell Voltage Clamp Technique -- 3.7 Recording the Pacemaker Current If -- 3.8 Recording Gap Junction Currents -- 3.9 Recording Monophasic Action Potentials -- 3.10 Heart on a Chip ... Extracellular Multielectrode Recordings from Cardiac Myocytes in Vitro -- 4 Histological Techniques -- 4.1 Immunohistochemistry for Structural and Functional Analysis in Cardiovascular Research -- 4.2 Classical Histological Staining Procedures in Cardiovascular Research -- 4.3 Practical Use of in situ Hybridisation and RT in situ PCR in Cardiovascular Research -- 4.4 Practical Use of Transmission Electron Microscopy for Analysis of Structures and Molecules in Cardiovascular Research -- 4.5 DAF Technique for Real-Time NO Imaging in the Human Myocardium -- 5 Cell Culture Techniques -- 5.1 Isolation and Culture of Adult Ventricular Cardiomyocytes -- 5.2 Culture of Neonatal Cardiomyocytes -- 5.3 Culture of Embryoid Bodies -- 5.4 Culturing and Differentiation of Embryonic and Adult Stem Cells for Heart Research and Transplantation Therapy -- 5.5 Preparation of Endothelial Cells from Micro- and Macrovascular Origin -- 5.6 In Vitro Cultivation of Vascular Smooth Muscle Cells -- 5.7 Engineering Heart Tissue for In Vitro and In Vivo Studies -- 6 Biochemical and Analytical Techniques -- 6.1 High-Performance Liquid Chromatography (HPLC) -- 6.2 Protein Biochemistry -- 6.3 Receptor and Binding Studies -- 6.4 Recording and Analysing Concentration-Response Curves -- 6.5 Measurement of NO and Endothelial Function in Cardiovascular Research -- 6.6 Biomechanical Stimulation of Vascular Cells In Vitro -- 6.7 Measurement of Function and Regulation of Adrenergic Receptors -- 6.8 Measurement of Function and Regulation of Muscarinic Acetylcholine Receptors -- 6.9 Cytometry in Cardiovascular Research -- 7 Molecular Biology Techniques -- 7.1 Real-Time PCR Analysis of Cardiac Samples -- 7.2 Use of cDNA Arrays to Explore Gene Expression in Genetically Manipulated Mice and Cell Lines -- 7.3 Using Antibody Arrays to Detect Protein-Protein Interactions -- 7.4 Surface Plasmon Resonance as a Method to Study the Kinetics and Amplitude of Protein-Protein Binding -- 7.5 How to Solve a Protein Structure by
Dimensions
unknown
File format
multiple file formats
Isbn
9783540265740
Level of compression
uncompressed
Quality assurance targets
absent
Reformatting quality
access
Specific material designation
remote

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